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. 1974 Dec;71(12):4688–4692. doi: 10.1073/pnas.71.12.4688

Localization of Escherichia coli Ribosomal Proteins S4 and S14 by Electron Microscopy of Antibody-Labeled Subunits

James A Lake *, Maryanne Pendergast *, Lawrence Kahan †, Masayasu Nomura ‡
PMCID: PMC433961  PMID: 4140507

Abstract

Binding sites for antibodies specific for proteins S4 and S14 of the small subunit of E. coli ribosomes have been mapped on the surface of the subunit by electron microscopy. Antibody binding to reconstituted subunits was shown to depend specifically on the presence of E. coli S4 and S14. Anti-S14 IgG was found to bind to a limited region of the ribosome surface. In contrast anti-S4 IgG was found to bind to three separated regions of the ribosome surface, suggesting S4 has an elongated conformation in situ.

Keywords: Bacillus stearothermophilus, IgG antibody, ribosome topography, reconstitution

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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