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. 1992 Jul 1;89(13):6085–6089. doi: 10.1073/pnas.89.13.6085

High rates of Ac/Ds germinal transposition in Arabidopsis suitable for gene isolation by insertional mutagenesis.

C Grevelding 1, D Becker 1, R Kunze 1, A von Menges 1, V Fantes 1, J Schell 1, R Masterson 1
PMCID: PMC49442  PMID: 1321434

Abstract

Overexpression of the Activator (Ac) transposase gene in Arabidopsis thaliana resulted in a minimal germinal transposition frequency of 27% in which independent Dissociation (Ds) transposition events were observed. Molecular analysis of 45 F1 generation Ac/Ds plants indicated that high rates of somatic excision had occurred, and independent germinal insertions were identified in F2 generation progeny plants. A tandem cauliflower mosaic virus (CaMV) promoter fused to two different Ac coding sequences significantly increased the rate of Ds transposition. The CaMV-Ac fusions activated single and multiple copies of two different Ds elements, DsDHFR and Ds35S-1, and reciprocal crosses resulted in similar transposition frequencies. The improved rate of independent germinal transposition observed makes Arabidopsis an ideal system for insertional mutagenesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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