Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1992 Apr;45(4):324–327. doi: 10.1136/jcp.45.4.324

Restriction endonuclease analysis of Aspergillus fumigatus DNA.

J P Burnie 1, A Coke 1, R C Matthews 1
PMCID: PMC495273  PMID: 1349614

Abstract

AIMS: To develop a genome based DNA fingerprinting system for Aspergillus fumigatus mould. METHODS: DNA was extracted from 21 isolates obtained from eight patients with an aspergilloma. This was with a freeze-dried mycelial extract fragmented in liquid nitrogen. DNA was subsequently purified by phenol-chloroform extraction followed by ultracentrifugation on a caesium chloride gradient. The DNA was restricted by EcoRI and Xba I. RESULTS: All isolates were identical when cut by EcoRI; Xba I delineated six DNA types. CONCLUSIONS: DNA fingerprinting can be used to type isolates of A fumigatus. Strains from within an aspergilloma which were morphologically distinct could either have the identical DNA fingerprint or produce a unique type.

Full text

PDF
325

Images in this article

325Image
on p.325
325Image
on p.325
326Image
on p.326

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burnie J. P., Matthews R. C., Clark I., Milne L. J. Immunoblot fingerprinting Aspergillus fumigatus. J Immunol Methods. 1989 Mar 31;118(2):179–186. doi: 10.1016/0022-1759(89)90004-5. [DOI] [PubMed] [Google Scholar]
  2. Carruba G., Pontieri E., De Bernardis F., Martino P., Cassone A. DNA fingerprinting and electrophoretic karyotype of environmental and clinical isolates of Candida parapsilosis. J Clin Microbiol. 1991 May;29(5):916–922. doi: 10.1128/jcm.29.5.916-922.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Denning D. W., Clemons K. V., Hanson L. H., Stevens D. A. Restriction endonuclease analysis of total cellular DNA of Aspergillus fumigatus isolates of geographically and epidemiologically diverse origin. J Infect Dis. 1990 Nov;162(5):1151–1158. doi: 10.1093/infdis/162.5.1151. [DOI] [PubMed] [Google Scholar]
  4. Dewhurst A. G., Cooper M. J., Khan S. M., Pallett A. P., Dathan J. R. Invasive aspergillosis in immunosuppressed patients: potential hazard of hospital building work. BMJ. 1990 Oct 6;301(6755):802–804. doi: 10.1136/bmj.301.6755.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doebbeling B. N., Hollis R. J., Isenberg H. D., Wenzel R. P., Pfaller M. A. Restriction fragment analysis of a Candida tropicalis outbreak of sternal wound infections. J Clin Microbiol. 1991 Jun;29(6):1268–1270. doi: 10.1128/jcm.29.6.1268-1270.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lee W., Burnie J. P., Matthews R. C., Oppenheim B. O., Damani N. N. Hospital outbreaks with yeasts. J Hosp Infect. 1991 Jun;18 (Suppl A):237–249. doi: 10.1016/0195-6701(91)90029-8. [DOI] [PubMed] [Google Scholar]
  7. Leslie C. E., Flannigan B., Milne L. J. Morphological studies on clinical isolates of Aspergillus fumigatus. J Med Vet Mycol. 1988;26(6):335–341. [PubMed] [Google Scholar]
  8. Matthews R., Burnie J. Assessment of DNA fingerprinting for rapid identification of outbreaks of systemic candidiasis. BMJ. 1989 Feb 11;298(6670):354–357. doi: 10.1136/bmj.298.6670.354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Opal S. M., Asp A. A., Cannady P. B., Jr, Morse P. L., Burton L. J., Hammer P. G., 2nd Efficacy of infection control measures during a nosocomial outbreak of disseminated aspergillosis associated with hospital construction. J Infect Dis. 1986 Mar;153(3):634–637. doi: 10.1093/infdis/153.3.634. [DOI] [PubMed] [Google Scholar]
  10. Oxford J. S. What is the true nature of epidemic influenza virus and how do new epidemic viruses spread? Epidemiol Infect. 1987 Aug;99(1):1–3. doi: 10.1017/s095026880006684x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Scherer S., Stevens D. A. Application of DNA typing methods to epidemiology and taxonomy of Candida species. J Clin Microbiol. 1987 Apr;25(4):675–679. doi: 10.1128/jcm.25.4.675-679.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Specht C. A., DiRusso C. C., Novotny C. P., Ullrich R. C. A method for extracting high-molecular-weight deoxyribonucleic acid from fungi. Anal Biochem. 1982 Jan 1;119(1):158–163. doi: 10.1016/0003-2697(82)90680-7. [DOI] [PubMed] [Google Scholar]
  13. Vincent R. D., Goewert R., Goldman W. E., Kobayashi G. S., Lambowitz A. M., Medoff G. Classification of Histoplasma capsulatum isolates by restriction fragment polymorphisms. J Bacteriol. 1986 Mar;165(3):813–818. doi: 10.1128/jb.165.3.813-818.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wöstemeyer J. Strain-dependent variation in ribosomal DNA arrangement in Absidia glauca. Eur J Biochem. 1985 Jan 15;146(2):443–448. doi: 10.1111/j.1432-1033.1985.tb08671.x. [DOI] [PubMed] [Google Scholar]
  15. de Bièvre C., Dauguet C., Nguyen V. H., Ibrahim-Granet O. Polymorphism in mitochondrial DNA of several Trichophyton rubrum isolates from clinical specimens. Ann Inst Pasteur Microbiol. 1987 Nov-Dec;138(6):719–727. doi: 10.1016/0769-2609(87)90149-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES