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. 1992 Apr;45(4):324–327. doi: 10.1136/jcp.45.4.324

Restriction endonuclease analysis of Aspergillus fumigatus DNA.

J P Burnie 1, A Coke 1, R C Matthews 1
PMCID: PMC495273  PMID: 1349614

Abstract

AIMS: To develop a genome based DNA fingerprinting system for Aspergillus fumigatus mould. METHODS: DNA was extracted from 21 isolates obtained from eight patients with an aspergilloma. This was with a freeze-dried mycelial extract fragmented in liquid nitrogen. DNA was subsequently purified by phenol-chloroform extraction followed by ultracentrifugation on a caesium chloride gradient. The DNA was restricted by EcoRI and Xba I. RESULTS: All isolates were identical when cut by EcoRI; Xba I delineated six DNA types. CONCLUSIONS: DNA fingerprinting can be used to type isolates of A fumigatus. Strains from within an aspergilloma which were morphologically distinct could either have the identical DNA fingerprint or produce a unique type.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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