Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1982 Feb;35(2):215–218. doi: 10.1136/jcp.35.2.215

Failure of the fluorescent antibody reaction to identify penicillinase-producing gonococci.

S A Waitkins, R D Anderson
PMCID: PMC497501  PMID: 6802878

Abstract

The fluorescent antibody test is now widely used to confirm the identity of Neisseria gonorrhoeae but may fail to identify penicillinase-producing strains (PPNG). This problem arises when conjugates are used that incorporate only gonococci that are not penicillinase-producers. We have shown that conjugates prepared from mixtures of PPNG and non-penicillinase producing gonococci give good fluorescent reactions. This difference in the reactions of PPNG strains is clearly related to their penicillinase-producing abilities, further study of the antigenic relation between penicillinase production and the antigenic structure of N gonorrhoeae is evidently required.

Full text

PDF
218

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashford W. A., Golash R. G., Hemming V. G. Penicillinase-producing Neisseria gonorrhoeae. Lancet. 1976 Sep 25;2(7987):657–658. doi: 10.1016/s0140-6736(76)92467-3. [DOI] [PubMed] [Google Scholar]
  2. DANIELSSON D. THE DEMONSTRATION OF N. GONORRHOEAE WITH THE AID OF FLUORESCENT ANTIBODIES. 1. IMMUNOLOGICAL STUDIES OF ANTIGONOCOCCAL SERA AND THEIR FLUORESCEIN-LABELLED GLOBULINS, WITH PARTICULAR REGARD TO SPECIFICITY. Acta Derm Venereol. 1963;43:451–464. [PubMed] [Google Scholar]
  3. Flynn J., Waitkins S. A. A serum-free medium for testing fermentation reactions in Neisseria gonorrhoeae. J Clin Pathol. 1972 Jun;25(6):525–527. doi: 10.1136/jcp.25.6.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lind I. Identification of Neisseria gonorrhoeae by means of fluorescent antibody technique. Acta Pathol Microbiol Scand. 1967;70(4):613–629. doi: 10.1111/j.1699-0463.1967.tb01330.x. [DOI] [PubMed] [Google Scholar]
  5. Lind I. Methodologic aspects of routine procedures for identification of Neisseria gonorrhoeae by immunofluorescence. Ann N Y Acad Sci. 1975 Jun 30;254:400–406. doi: 10.1111/j.1749-6632.1975.tb29191.x. [DOI] [PubMed] [Google Scholar]
  6. O'Callaghan C. H., Morris A., Kirby S. M., Shingler A. H. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283–288. doi: 10.1128/aac.1.4.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Penicillinase-producing Neisseria gonorrhoeae. Lancet. 1976 Oct 9;2(7989):793–793. [PubMed] [Google Scholar]
  8. Percival A., Rowlands J., Corkill J. E., Alergant C. D., Arya O. P., Rees E., Annels E. H. Penicillinase-producing Gonococci in Liverpool. Lancet. 1976 Dec 25;2(8000):1379–1382. doi: 10.1016/s0140-6736(76)91919-x. [DOI] [PubMed] [Google Scholar]
  9. Piot P., Roberts M., Ninane G. Beta-lactamase production in commensal Neisseriaceae. Lancet. 1979 Mar 17;1(8116):619–619. doi: 10.1016/s0140-6736(79)91061-4. [DOI] [PubMed] [Google Scholar]
  10. Seth A. Use of trimethoprim to prevent overgrowth by Proteus in the cultivation of N. gonorrhoeae. Br J Vener Dis. 1970 Jun;46(3):201–202. doi: 10.1136/sti.46.3.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. THAYER J. D., MARTIN J. E., Jr A SELECTIVE MEDIUM FOR THE CULTIVATION OF N. GONORRHOEAE AND N. MENINGITIDIS. Public Health Rep. 1964 Jan;79:49–57. [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES