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Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1999 Oct;52(10):766–769. doi: 10.1136/jcp.52.10.766

Rapid detection of the factor V Leiden (1691 G > A) and haemochromatosis (845 G > A) mutation by fluorescence resonance energy transfer (FRET) and real time PCR.

S H Neoh 1, M J Brisco 1, F A Firgaira 1, K J Trainor 1, D R Turner 1, A A Morley 1
PMCID: PMC501573  PMID: 10674036

Abstract

A rapid method based on fluorescence resonance energy transfer (FRET) and real time polymerase chain reaction (PCR) was used to identify the haemochromatosis genotype in 112 individuals and the factor V genotype in 134 individuals. The results were compared with conventional methods based on restriction enzyme digestion of PCR products. The two methods agreed in 244 of the 246 individuals; for the other two individuals, sequencing showed that they had been incorrectly genotyped by the standard method but correctly genotyped by FRET. The simplicity, speed, and accuracy of real time PCR analysis using FRET probes make it the method of choice in the clinical laboratory for genotyping the haemochromatosis and factor V genes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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