Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1977 Apr;22(1):23–30. doi: 10.1128/jvi.22.1.23-30.1977

Inhibition of bacteriophage M13 and phix174 duplex DNA replication and single-strand synthesis in temperature-sensitive dnaZ mutants of Escherichia coli.

W G Haldenwang, J R Walker
PMCID: PMC515682  PMID: 323515

Abstract

A functional dnaZ product, known to be essential for host DNA polymerization and for the synthesis of M13 and phiX174 parental replicative-form (RF) DNA, is required also for RF replication and single-strand synthesis by both of these phages. All three stages of M13 and phiX174DNA replication (parental RF formation, RF replication, and single-strand synthesis) are inhibited in dnazts mutants at elevated temperatures. In addition, the thermolabile step in M13 parental RF formation appears to occur after RNA priming;i.e., the synthesis of M13 RF DNA proceeded when a dnaZts mutant, infected at a nonpermissive temperature, was transferred to a permissive temperature in the presence of rifampin.

Full text

PDF
28

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bouché J. P., Zechel K., Kornberg A. dnaG gene product, a rifampicin-resistant RNA polymerase, initiates the conversion of a single-stranded coliphage DNA to its duplex replicative form. J Biol Chem. 1975 Aug 10;250(15):5995–6001. [PubMed] [Google Scholar]
  2. Brown L. R., Dowell C. E. Replication of coliphage M-13. II. Intracellular deoxyribonucleic acid forms associated with M-13 infection of mitomycin C-treated cells. J Virol. 1968 Nov;2(11):1296–1307. doi: 10.1128/jvi.2.11.1296-1307.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dumas L. B., Miller C. A., Bayne M. L. Rifampin inhibition of bacteriophage phiX174 parental replicative-form DNA synthesis in an Escherichia coli dnaC mutant. J Virol. 1975 Sep;16(3):575–580. doi: 10.1128/jvi.16.3.575-580.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dumas L. B., Miller C. A. Replication of bacteriophage phiX174 DNA in a temperature-sensitive dnaE mutant of Escherichia coli C. J Virol. 1973 Jun;11(6):848–855. doi: 10.1128/jvi.11.6.848-855.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fidanián H. M., Ray D. S. Replication of bacteriophage M13. 8. Differential effects of rifampicin and nalidixic acid on the synthesis of the two strands of M13 duplex DNA. J Mol Biol. 1974 Feb 15;83(1):63–82. doi: 10.1016/0022-2836(74)90424-0. [DOI] [PubMed] [Google Scholar]
  6. Fidanián H. M., Ray D. S. Replication of bacteriophage M13. VII. Requirement of the gene 2 protein for the accumulation of a specific RFII species. J Mol Biol. 1972 Dec 14;72(1):51–63. doi: 10.1016/0022-2836(72)90067-8. [DOI] [PubMed] [Google Scholar]
  7. GUTHRIE G. D., SINSHEIMER R. L. Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174. Biochim Biophys Acta. 1963 Jun 25;72:290–297. [PubMed] [Google Scholar]
  8. Geider K. Molecular aspects of DNA replication in Escherichia coli systems. Curr Top Microbiol Immunol. 1976;74:55–112. doi: 10.1007/978-3-642-66336-9_3. [DOI] [PubMed] [Google Scholar]
  9. Greenlee L. L. Replication of bacteriophage phiX-174 in a mutant of Escherichia coli defective in the dnaE gene. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1757–1760. doi: 10.1073/pnas.70.6.1757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Haldenwang W. G., Walker J. R. Parental RF formation on phages phiX174 and M13 requires the dnaZ gene product of Escherichia coli. Biochem Biophys Res Commun. 1976 Jun 7;70(3):932–938. doi: 10.1016/0006-291x(76)90681-1. [DOI] [PubMed] [Google Scholar]
  11. Kranias E. G., Dumas L. B. Replication of bacteriophage phiX174 DNA in a temperature-sensitive dnaC mutant of escherichia coli C. J Virol. 1974 Jan;13(1):146–154. doi: 10.1128/jvi.13.1.146-154.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Marvin D. A., Hohn B. Filamentous bacterial viruses. Bacteriol Rev. 1969 Jun;33(2):172–209. doi: 10.1128/br.33.2.172-209.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McFadden G., Denhardt D. T. Mechanism of replication of phi x174 single-stranded DNA. IX. Requirement for the Escherichia coli dnaG protein. J Virol. 1974 Nov;14(5):1070–1075. doi: 10.1128/jvi.14.5.1070-1075.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ray D. S., Dueber J., Suggs S. Replication of bacteriophage M13 IX. Requirement of the Escherichia coli dnaG function for M13 duplex DNA replication. J Virol. 1975 Aug;16(2):348–355. doi: 10.1128/jvi.16.2.348-355.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ray D. S. Replication of bacteriophage M13. IV. Synthesis of M13-specific DNA in the presence of chloramphenicol. J Mol Biol. 1970 Oct 28;53(2):239–250. doi: 10.1016/0022-2836(70)90297-4. [DOI] [PubMed] [Google Scholar]
  16. Schekman R., Weiner A., Kornberg A. Multienzyme systems of DNA replication. Science. 1974 Dec 13;186(4168):987–993. doi: 10.1126/science.186.4168.987. [DOI] [PubMed] [Google Scholar]
  17. Sinsheimer R. L. Bacteriophage phi-X174 and related viruses. Prog Nucleic Acid Res Mol Biol. 1968;8:115–169. [PubMed] [Google Scholar]
  18. Staudenbauer W. L. Involvement of DNA polymerases I and III in the replication of bacteriophage M-13. Eur J Biochem. 1974 Nov 1;49(1):249–256. doi: 10.1111/j.1432-1033.1974.tb03829.x. [DOI] [PubMed] [Google Scholar]
  19. Truitt C. L., Walker J. R. Growth of phages lambda, phiX174, and Ml3 requires the dnaZ (previously dnaH) gene product of Escherichia coli. Biochem Biophys Res Commun. 1974 Dec 11;61(3):1036–1042. doi: 10.1016/0006-291x(74)90259-9. [DOI] [PubMed] [Google Scholar]
  20. Wickner S., Hurwitz J. Involvement of escherichia coli dnaZ gene product in DNA elongation in vitro. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1053–1057. doi: 10.1073/pnas.73.4.1053. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wickner W., Schekman R., Geider K., Kornberg A. A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1764–1767. doi: 10.1073/pnas.70.6.1764. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES