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. 1977 Apr;22(1):23–30. doi: 10.1128/jvi.22.1.23-30.1977

Inhibition of bacteriophage M13 and phix174 duplex DNA replication and single-strand synthesis in temperature-sensitive dnaZ mutants of Escherichia coli.

W G Haldenwang, J R Walker
PMCID: PMC515682  PMID: 323515

Abstract

A functional dnaZ product, known to be essential for host DNA polymerization and for the synthesis of M13 and phiX174 parental replicative-form (RF) DNA, is required also for RF replication and single-strand synthesis by both of these phages. All three stages of M13 and phiX174DNA replication (parental RF formation, RF replication, and single-strand synthesis) are inhibited in dnazts mutants at elevated temperatures. In addition, the thermolabile step in M13 parental RF formation appears to occur after RNA priming;i.e., the synthesis of M13 RF DNA proceeded when a dnaZts mutant, infected at a nonpermissive temperature, was transferred to a permissive temperature in the presence of rifampin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bouché J. P., Zechel K., Kornberg A. dnaG gene product, a rifampicin-resistant RNA polymerase, initiates the conversion of a single-stranded coliphage DNA to its duplex replicative form. J Biol Chem. 1975 Aug 10;250(15):5995–6001. [PubMed] [Google Scholar]
  2. Brown L. R., Dowell C. E. Replication of coliphage M-13. II. Intracellular deoxyribonucleic acid forms associated with M-13 infection of mitomycin C-treated cells. J Virol. 1968 Nov;2(11):1296–1307. doi: 10.1128/jvi.2.11.1296-1307.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dumas L. B., Miller C. A., Bayne M. L. Rifampin inhibition of bacteriophage phiX174 parental replicative-form DNA synthesis in an Escherichia coli dnaC mutant. J Virol. 1975 Sep;16(3):575–580. doi: 10.1128/jvi.16.3.575-580.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dumas L. B., Miller C. A. Replication of bacteriophage phiX174 DNA in a temperature-sensitive dnaE mutant of Escherichia coli C. J Virol. 1973 Jun;11(6):848–855. doi: 10.1128/jvi.11.6.848-855.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fidanián H. M., Ray D. S. Replication of bacteriophage M13. 8. Differential effects of rifampicin and nalidixic acid on the synthesis of the two strands of M13 duplex DNA. J Mol Biol. 1974 Feb 15;83(1):63–82. doi: 10.1016/0022-2836(74)90424-0. [DOI] [PubMed] [Google Scholar]
  6. Fidanián H. M., Ray D. S. Replication of bacteriophage M13. VII. Requirement of the gene 2 protein for the accumulation of a specific RFII species. J Mol Biol. 1972 Dec 14;72(1):51–63. doi: 10.1016/0022-2836(72)90067-8. [DOI] [PubMed] [Google Scholar]
  7. GUTHRIE G. D., SINSHEIMER R. L. Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174. Biochim Biophys Acta. 1963 Jun 25;72:290–297. [PubMed] [Google Scholar]
  8. Geider K. Molecular aspects of DNA replication in Escherichia coli systems. Curr Top Microbiol Immunol. 1976;74:55–112. doi: 10.1007/978-3-642-66336-9_3. [DOI] [PubMed] [Google Scholar]
  9. Greenlee L. L. Replication of bacteriophage phiX-174 in a mutant of Escherichia coli defective in the dnaE gene. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1757–1760. doi: 10.1073/pnas.70.6.1757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Haldenwang W. G., Walker J. R. Parental RF formation on phages phiX174 and M13 requires the dnaZ gene product of Escherichia coli. Biochem Biophys Res Commun. 1976 Jun 7;70(3):932–938. doi: 10.1016/0006-291x(76)90681-1. [DOI] [PubMed] [Google Scholar]
  11. Kranias E. G., Dumas L. B. Replication of bacteriophage phiX174 DNA in a temperature-sensitive dnaC mutant of escherichia coli C. J Virol. 1974 Jan;13(1):146–154. doi: 10.1128/jvi.13.1.146-154.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Marvin D. A., Hohn B. Filamentous bacterial viruses. Bacteriol Rev. 1969 Jun;33(2):172–209. doi: 10.1128/br.33.2.172-209.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McFadden G., Denhardt D. T. Mechanism of replication of phi x174 single-stranded DNA. IX. Requirement for the Escherichia coli dnaG protein. J Virol. 1974 Nov;14(5):1070–1075. doi: 10.1128/jvi.14.5.1070-1075.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ray D. S., Dueber J., Suggs S. Replication of bacteriophage M13 IX. Requirement of the Escherichia coli dnaG function for M13 duplex DNA replication. J Virol. 1975 Aug;16(2):348–355. doi: 10.1128/jvi.16.2.348-355.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ray D. S. Replication of bacteriophage M13. IV. Synthesis of M13-specific DNA in the presence of chloramphenicol. J Mol Biol. 1970 Oct 28;53(2):239–250. doi: 10.1016/0022-2836(70)90297-4. [DOI] [PubMed] [Google Scholar]
  16. Schekman R., Weiner A., Kornberg A. Multienzyme systems of DNA replication. Science. 1974 Dec 13;186(4168):987–993. doi: 10.1126/science.186.4168.987. [DOI] [PubMed] [Google Scholar]
  17. Sinsheimer R. L. Bacteriophage phi-X174 and related viruses. Prog Nucleic Acid Res Mol Biol. 1968;8:115–169. [PubMed] [Google Scholar]
  18. Staudenbauer W. L. Involvement of DNA polymerases I and III in the replication of bacteriophage M-13. Eur J Biochem. 1974 Nov 1;49(1):249–256. doi: 10.1111/j.1432-1033.1974.tb03829.x. [DOI] [PubMed] [Google Scholar]
  19. Truitt C. L., Walker J. R. Growth of phages lambda, phiX174, and Ml3 requires the dnaZ (previously dnaH) gene product of Escherichia coli. Biochem Biophys Res Commun. 1974 Dec 11;61(3):1036–1042. doi: 10.1016/0006-291x(74)90259-9. [DOI] [PubMed] [Google Scholar]
  20. Wickner S., Hurwitz J. Involvement of escherichia coli dnaZ gene product in DNA elongation in vitro. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1053–1057. doi: 10.1073/pnas.73.4.1053. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wickner W., Schekman R., Geider K., Kornberg A. A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1764–1767. doi: 10.1073/pnas.70.6.1764. [DOI] [PMC free article] [PubMed] [Google Scholar]

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