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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1991 May 15;88(10):4468–4471. doi: 10.1073/pnas.88.10.4468

Free-flow electrophoresis with trapping by a transverse inhomogeneous field.

A Ajdari 1, J Prost 1
PMCID: PMC51681  PMID: 2034684

Abstract

We propose an electrophoretic technique combining the use of a series of dielectric traps controlled by an ac electric field and conventional continuous field free-flow electrophoresis. From the theoretical model that we describe, one can expect, for DNA electrophoresis, an improvement of one to two orders of magnitude in the selectivities or experimental durations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bier F. F., Bettag U., Rheingans T., Adrian H., Barths J., Hausmann M., Bühring H. J., Rohwer P., Dölle J., Cremer C. Determination of the electrophoretic mobility of chromosomes by free flow electrophoresis. I. Morphology and stability. Electrophoresis. 1989 Oct;10(10):690–697. doi: 10.1002/elps.1150101006. [DOI] [PubMed] [Google Scholar]
  2. Cantor C. R., Smith C. L., Mathew M. K. Pulsed-field gel electrophoresis of very large DNA molecules. Annu Rev Biophys Biophys Chem. 1988;17:287–304. doi: 10.1146/annurev.bb.17.060188.001443. [DOI] [PubMed] [Google Scholar]
  3. Schwartz D. C., Cantor C. R. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. doi: 10.1016/0092-8674(84)90301-5. [DOI] [PubMed] [Google Scholar]
  4. Stevens F. J. Fractionation of macromolecules in an alternating transverse electric field: simulation of the method. J Biochem Biophys Methods. 1990 Apr-May;20(4):275–292. doi: 10.1016/0165-022x(90)90090-y. [DOI] [PubMed] [Google Scholar]
  5. Ulanovsky L., Drouin G., Gilbert W. DNA trapping electrophoresis. Nature. 1990 Jan 11;343(6254):190–192. doi: 10.1038/343190a0. [DOI] [PubMed] [Google Scholar]
  6. Zimm BH. Size flucuations can explain anomalous mobility in field-inversion electrophoresis of DNA. Phys Rev Lett. 1988 Dec 26;61(26):2965–2968. doi: 10.1103/PhysRevLett.61.2965. [DOI] [PubMed] [Google Scholar]

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