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. 1991 Dec 1;88(23):10392–10396. doi: 10.1073/pnas.88.23.10392

In vitro assembly of apophytochrome and apophytochrome deletion mutants expressed in yeast with phycocyanobilin.

L Deforce 1, K Tomizawa 1, N Ito 1, D Farrens 1, P S Song 1, M Furuya 1
PMCID: PMC52934  PMID: 1961705

Abstract

Recombinant pea type I phytochrome apoprotein expressed in yeast is shown to assemble in vitro with phycocyanobilin to produce a photoreversible phytochrome-like adduct. As an initial investigation of the amino acid sequence requirements for chromophore incorporation, three phyA gene product deletion mutants were produced in yeast. Truncation of the N-terminal tail to residue 46 demonstrates that this region is not critical to bilin attachment, but a deletion mutant lacking 222 amino acids from the N terminus failed to yield holophytochrome in vitro, under the same conditions. A mutant comprising a deletion of the C terminus to residue 548 showed bilin incorporation and red/far-red photoreversibility, indicating that bilin-apophytochrome assembly still occurred even when the entire C-terminal domain was truncated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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