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. 1976 Aug;58(2):156–162. doi: 10.1104/pp.58.2.156

Stimulation of Carbon Dioxide Fixation in Isolated Pea Chloroplasts by Catalytic Amounts of Adenine Nucleotides 1

Simon P Robinson a, Joseph T Wiskich a
PMCID: PMC542203  PMID: 16659638

Abstract

Carbon dioxide-dependent O2 evolution by isolated pea (Pisum sativum var. Massey Gem) chloroplasts was increased two to 12 times by the addition of ATP. O2 evolution was also stimulated by ADP and to a lesser extent by AMP. The ATP effects were not due to broken chloroplasts present in the preparations nor was ATP acting as a phosphate source. We concluded that the adenine nucleotides were acting catalytically. The concentration of ATP required for half-maximum rate of O2 evolution was 16 to 25 μm. The degree to which ATP stimulated O2 evolution depended on the age of pea plants from which the chloroplasts were isolated. Spinach (Spinacia oleracea var. True Hybrid 102) chloroplasts did not show a consistent stimulation of O2 evolution by adenine nucleotides.

The adenine nucleotide content of pea chloroplasts was not lower than that of spinach chloroplasts, but pea chloroplasts which showed a large stimulation of O2 evolution by ATP contained an ATP-hydrolyzing reaction with rates of 10 to 50 μmol ATP hydrolyzed mg chlorophyll−1 hour−1. The rate of the ATP-consuming reaction was much lower in spinach chloroplasts and in chloroplasts from older pea plants which did not show large stimulation of O2 evolution by ATP. We propose that the ATP-consuming reaction, with a high affinity for ATP, decreased the effective size of the ATP pool available for CO2 fixation. Added adenine nucleotides could be transported into the chloroplasts increasing the concentration of internal nucleotides. Calculations showed that the adenine nucleotide transporter on the outer chloroplast membranes could operate at a sufficient rate to produce such an effect.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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