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. 1967 Sep;15(5):1145–1149. doi: 10.1128/am.15.5.1145-1149.1967

Immunofluorescence Technique for the Detection of Salmonellae in Various Foods

N F Insalata 1, S J Schulte 1, J H Berman 1,1
PMCID: PMC547156  PMID: 16349728

Abstract

Salmonella species have been detected in nine food varieties by use of fluorescent antibodies without false-positive or false-negative results. Test antisera were specially prepared, commercially available, conjugated polyvalent O globulin absorbed with cultures of Escherichia coli and Citrobacter freundii, and polyvalent phase II H globulin antibodies. Use of this technique permits a decrease of 24 hr in time normally required for Salmonella detection when compared with cultural Salmonella recovery methods.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Caldwell W. J., Stulberg C. S., Peterson W. D., Jr Somatic and flagellar immunofluorescence of Salmonella. J Bacteriol. 1966 Oct;92(4):1177–1187. doi: 10.1128/jb.92.4.1177-1187.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. GEORGALA D. L., BOOTHROYD M. A RAPID IMMUNOFLUORESCENCE TECHNIQUE FOR DETECTING SALMONELLAE IN RAW MEAT. J Hyg (Lond) 1964 Sep;62:319–327. doi: 10.1017/s0022172400040031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Georgala D. L., Boothroyd M., Hayes P. R. Further evaluation of a rapid immunofluorescence technique for detecting salmonellae in meat and poultry. J Appl Bacteriol. 1965 Dec;28(3):421–425. doi: 10.1111/j.1365-2672.1965.tb02172.x. [DOI] [PubMed] [Google Scholar]
  4. HAGLUND J. R., AYRES J. C., PATON A. M., KRAFT A. A., QUINN L. Y. DETECTION OF SALMONELLA IN EGGS AND EGG PRODUCTS WITH FLUORESCENT ANTIBODY. Appl Microbiol. 1964 Sep;12:447–450. doi: 10.1128/am.12.5.447-450.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. NORTH W. R., Jr Lactose pre-enrichment method for isolation of Samonella from dried egg albumen. Its use in a survery of commercially produced albumen. Appl Microbiol. 1961 May;9:188–195. doi: 10.1128/am.9.3.188-195.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. NORTH W. R., Jr Use of crystal violet or brilliant green dyes for the determination of Salmonellae in dried food products. J Bacteriol. 1960 Dec;80:861–861. doi: 10.1128/jb.80.6.861-861.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. THOMASON B. M., CHERRY W. B., EDWARDS P. R. Staining bacterial smears with fluorescent antibody. VI. Identification of Salmonellae in fecal specimens. J Bacteriol. 1959 Apr;77(4):478–486. doi: 10.1128/jb.77.4.478-486.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. THOMASON B. M., CHERRY W. B., MOODY M. D. Staining bacterial smears with fluorescent antibody. III. Antigenic analysis of Salmonella typhosa by means of fluorescent antibody and agglutination reactions. J Bacteriol. 1957 Oct;74(4):525–532. doi: 10.1128/jb.74.4.525-532.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]

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