Abstract
Salmonella species have been detected in nine food varieties by use of fluorescent antibodies without false-positive or false-negative results. Test antisera were specially prepared, commercially available, conjugated polyvalent O globulin absorbed with cultures of Escherichia coli and Citrobacter freundii, and polyvalent phase II H globulin antibodies. Use of this technique permits a decrease of 24 hr in time normally required for Salmonella detection when compared with cultural Salmonella recovery methods.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Caldwell W. J., Stulberg C. S., Peterson W. D., Jr Somatic and flagellar immunofluorescence of Salmonella. J Bacteriol. 1966 Oct;92(4):1177–1187. doi: 10.1128/jb.92.4.1177-1187.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GEORGALA D. L., BOOTHROYD M. A RAPID IMMUNOFLUORESCENCE TECHNIQUE FOR DETECTING SALMONELLAE IN RAW MEAT. J Hyg (Lond) 1964 Sep;62:319–327. doi: 10.1017/s0022172400040031. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Georgala D. L., Boothroyd M., Hayes P. R. Further evaluation of a rapid immunofluorescence technique for detecting salmonellae in meat and poultry. J Appl Bacteriol. 1965 Dec;28(3):421–425. doi: 10.1111/j.1365-2672.1965.tb02172.x. [DOI] [PubMed] [Google Scholar]
- HAGLUND J. R., AYRES J. C., PATON A. M., KRAFT A. A., QUINN L. Y. DETECTION OF SALMONELLA IN EGGS AND EGG PRODUCTS WITH FLUORESCENT ANTIBODY. Appl Microbiol. 1964 Sep;12:447–450. doi: 10.1128/am.12.5.447-450.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NORTH W. R., Jr Lactose pre-enrichment method for isolation of Samonella from dried egg albumen. Its use in a survery of commercially produced albumen. Appl Microbiol. 1961 May;9:188–195. doi: 10.1128/am.9.3.188-195.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NORTH W. R., Jr Use of crystal violet or brilliant green dyes for the determination of Salmonellae in dried food products. J Bacteriol. 1960 Dec;80:861–861. doi: 10.1128/jb.80.6.861-861.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- THOMASON B. M., CHERRY W. B., EDWARDS P. R. Staining bacterial smears with fluorescent antibody. VI. Identification of Salmonellae in fecal specimens. J Bacteriol. 1959 Apr;77(4):478–486. doi: 10.1128/jb.77.4.478-486.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- THOMASON B. M., CHERRY W. B., MOODY M. D. Staining bacterial smears with fluorescent antibody. III. Antigenic analysis of Salmonella typhosa by means of fluorescent antibody and agglutination reactions. J Bacteriol. 1957 Oct;74(4):525–532. doi: 10.1128/jb.74.4.525-532.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]