Skip to main content
Applied Microbiology logoLink to Applied Microbiology
. 1968 Dec;16(12):1886–1891. doi: 10.1128/am.16.12.1886-1891.1968

Yields of Bacterial Cells from Hydrocarbons

Richard S Wodzinski 1, Marvin J Johnson 1
PMCID: PMC547790  PMID: 5726161

Abstract

A strain of Nocardia and one of Pseudomonas, both isolated on pristane (2,6,10,14-tetramethylpentadecane), gave cell yields of approximately 100% on n-octadecane and pristane. Both organisms grew more rapidly on the n-octadecane than on the pristane. A mixed culture, isolated on 3-methylheptane, whose two components were identified as species of Pseudomonas and of Nocardia, gave approximately 100% cell yields and grew with generation times of about 5 hr on n-heptane, n-octane, and 2-methylheptane. The generation time on 3-methylheptane was 8.6 hr and the cell yield was only 79%. A strain of Pseudomonas isolated from naphthalene enrichments and one from phenanthrene enrichments both gave a cell yield of 50% on naphthalene. The phenanthrene isolate gave a cell yield of 40% on phenanthrene. A Nocardia species isolated on benzene gave a 79% cell yield on benzene. The generation times of the bacteria isolated on aromatic hydrocarbons were related to the solubility of the aromatic hydrocarbons on which they were grown; the more insoluble hydrocarbons gave slower growth.

Full text

PDF
1888

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHASE H. H., DAVIS J. B., RAYMOND R. L. Mycobacterium paraffinicum n. sp., a bacterium isolated from soil. Appl Microbiol. 1956 Nov;4(6):310–315. doi: 10.1128/am.4.6.310-315.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DWORKIN M., FOSTER J. W. Studies on Pseudomonas methanica (Söhngen) nov. comb. J Bacteriol. 1956 Nov;72(5):646–659. doi: 10.1128/jb.72.5.646-659.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Foster J. W., Davis R. H. A methane-dependent coccus, with notes on classification and nomenclature of obligate, methane-utilizing bacteria. J Bacteriol. 1966 May;91(5):1924–1931. doi: 10.1128/jb.91.5.1924-1931.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. JOHNSON M. J. A rapid micromethod for estimation of non-volatile organic matter. J Biol Chem. 1949 Dec;181(2):707–711. [PubMed] [Google Scholar]
  5. RAYMOND R. L., DAVIS J. B. n-Alkane utilization and lipid formation by a Nocardia. Appl Microbiol. 1960 Nov;8:329–334. doi: 10.1128/am.8.6.329-334.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Vary P. S., Johnson M. J. Cell yields of bacteria grown on methane. Appl Microbiol. 1967 Nov;15(6):1473–1478. doi: 10.1128/am.15.6.1473-1478.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES