Abstract
A strain of Nocardia and one of Pseudomonas, both isolated on pristane (2,6,10,14-tetramethylpentadecane), gave cell yields of approximately 100% on n-octadecane and pristane. Both organisms grew more rapidly on the n-octadecane than on the pristane. A mixed culture, isolated on 3-methylheptane, whose two components were identified as species of Pseudomonas and of Nocardia, gave approximately 100% cell yields and grew with generation times of about 5 hr on n-heptane, n-octane, and 2-methylheptane. The generation time on 3-methylheptane was 8.6 hr and the cell yield was only 79%. A strain of Pseudomonas isolated from naphthalene enrichments and one from phenanthrene enrichments both gave a cell yield of 50% on naphthalene. The phenanthrene isolate gave a cell yield of 40% on phenanthrene. A Nocardia species isolated on benzene gave a 79% cell yield on benzene. The generation times of the bacteria isolated on aromatic hydrocarbons were related to the solubility of the aromatic hydrocarbons on which they were grown; the more insoluble hydrocarbons gave slower growth.
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Selected References
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- CHASE H. H., DAVIS J. B., RAYMOND R. L. Mycobacterium paraffinicum n. sp., a bacterium isolated from soil. Appl Microbiol. 1956 Nov;4(6):310–315. doi: 10.1128/am.4.6.310-315.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DWORKIN M., FOSTER J. W. Studies on Pseudomonas methanica (Söhngen) nov. comb. J Bacteriol. 1956 Nov;72(5):646–659. doi: 10.1128/jb.72.5.646-659.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Foster J. W., Davis R. H. A methane-dependent coccus, with notes on classification and nomenclature of obligate, methane-utilizing bacteria. J Bacteriol. 1966 May;91(5):1924–1931. doi: 10.1128/jb.91.5.1924-1931.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JOHNSON M. J. A rapid micromethod for estimation of non-volatile organic matter. J Biol Chem. 1949 Dec;181(2):707–711. [PubMed] [Google Scholar]
- RAYMOND R. L., DAVIS J. B. n-Alkane utilization and lipid formation by a Nocardia. Appl Microbiol. 1960 Nov;8:329–334. doi: 10.1128/am.8.6.329-334.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vary P. S., Johnson M. J. Cell yields of bacteria grown on methane. Appl Microbiol. 1967 Nov;15(6):1473–1478. doi: 10.1128/am.15.6.1473-1478.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]