Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1980 Jun;28(3):1033–1037. doi: 10.1128/iai.28.3.1033-1037.1980

Lysis and protoplast formation of group B streptococci by mutanolysin.

G B Calandra, R M Cole
PMCID: PMC551054  PMID: 6995317

Abstract

Group B streptococci, refractory to previously tested muralysins under physiological conditions, were successfully converted to protoplasts by use of a recently describede N-acetyl muramidase, mutanolysin, derived from a streptomycete. Purified enzyme was effective, but crude preparations, although degrading cell walls, simultaneously produced peculiar effects of cytoplasmic coagulation, retention of cell shape, loss of some intracellular enzymes, and a rise in optical density. Addition of purified mutanolysin to the array of muralysins (group C streptococcal phage-associated lysin, lysozyme), previously successful in preparing protoplasts of different streptococci, now makes possible enzymatic preparation of protoplasts of streptococci of groups A, B, C. D. G, and H.

Full text

PDF
1034

Images in this article

1035Fig. 1
on p.1035
1035Fig. 2
on p.1035
1035Fig. 3
on p.1035
1035Fig. 4
on p.1035
1035Fig. 5
on p.1035
1035Fig. 6
on p.1035
1035Fig. 7
on p.1035
1035Fig. 8
on p.1035

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Calandra G. B., Nugent K. M., Cole R. M. Preparation of protoplasts of group H streptococci (Streptococcus sanguis). Appl Microbiol. 1975 Jan;29(1):90–93. doi: 10.1128/am.29.1.90-93.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Calandra G. B., Oginsky E. L. Cellular streptolysin S-related hemolysins of group A Streptococcus C203S. Infect Immun. 1975 Jul;12(1):13–28. doi: 10.1128/iai.12.1.13-28.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Calandra G. B., Theodore T. S. Cellular location of streptolysin O. Infect Immun. 1975 Oct;12(4):750–753. doi: 10.1128/iai.12.4.750-753.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cole R. M., Popkin T. J., Boylan R. J., Mendelson N. H. Ultrastructure of a temperature-sensitive rod- mutant of Bacillus subtilis. J Bacteriol. 1970 Sep;103(3):793–810. doi: 10.1128/jb.103.3.793-810.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Coleman S. E., Van de Rijn I., Bleiweis A. S. Lysis of cariogenic and noncariogenic oral streptococci with lysozyme. J Dent Res. 1971 Jul-Aug;50(4):939–943. doi: 10.1177/00220345710500042601. [DOI] [PubMed] [Google Scholar]
  6. Coleman S. E., van de Rijn I., Bleiweis A. S. Lysis of grouped and ungrouped streptococci by lysozyme. Infect Immun. 1970 Nov;2(5):563–569. doi: 10.1128/iai.2.5.563-569.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fischetti V. A., Gotschlich E. C., Bernheimer A. W. Purification and physical properties of group C streptococcal phage-associated lysin. J Exp Med. 1971 May 1;133(5):1105–1117. doi: 10.1084/jem.133.5.1105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Glick A. D., Ranhand J. M., Cole R. M. Degradation of group A streptococcal cell walls by egg-white lysozyme and human lysosomal enzymes. Infect Immun. 1972 Sep;6(3):403–413. doi: 10.1128/iai.6.3.403-413.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hamada S., Torii M., Kotani S., Masuda N., Ooshima T., Yokogawa K., Kawata S. Lysis of Streptococcus mutans cells with mutanolysin, a lytic enzyme prepared from a culture liquor of Streptomyces globisporus 1829. Arch Oral Biol. 1978;23(7):543–549. doi: 10.1016/0003-9969(78)90268-6. [DOI] [PubMed] [Google Scholar]
  10. KRAUSE R. M., McCARTY M. Studies on the chemical structure of the streptococcal cell wall. II. The composition of group C cell walls and chemical basis for serologic specificity of the carbohydrate moiety. J Exp Med. 1962 Jan 1;115:49–62. doi: 10.1084/jem.115.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KRAUSE R. M. Studies on the bacteriophages of hemolytic streptococci. II. Antigens released from the streptococcal cell wall by a phage-associated lysin. J Exp Med. 1958 Dec 1;108(6):803–821. doi: 10.1084/jem.108.6.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]
  13. Yokogawa K., Kawata S., Nishimura S., Ikeda Y., Yoshimura Y. Mutanolysin, bacteriolytic agent for cariogenic Streptococci: partial purification and properties. Antimicrob Agents Chemother. 1974 Aug;6(2):156–165. doi: 10.1128/aac.6.2.156. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES