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. 1985 Jan;4(1):249–255. doi: 10.1002/j.1460-2075.1985.tb02343.x

Co-transcribed 3' host sequences augment expression of integrated hepatitis B virus DNA.

A F von Loringhoven, S Koch, P H Hofschneider, R Koshy
PMCID: PMC554177  PMID: 2990895

Abstract

We have previously reported the cloning and structural analysis of integrated hepatitis B virus DNA copies from the human hepatoma cell line PLC/PRF/5. Here we show that the cloned DNA fragments of 10.7 kb and 10.5 kb contain intact coding sequences for HBsAg since Ltk- cells transfected with these DNAs secrete considerable amounts of HBsAg. We show for the 10.7-kb fragment that multiple readthrough messages composed of viral as well as cellular sequences are transcribed. These RNAs differ only in their 3' sequences. Furthermore, the 10.7-kb insert leads to a substantial increase in HBsAg produced compared with HBV DNA and with the 10.5-kb insert. We provide evidence that the different 3' sequences on the HBsAg transcripts account for the augmentation of expression.

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