Skip to main content
PMC home page
The EMBO Journal logoLink to The EMBO Journal
. 1983;2(10):1801–1805. doi: 10.1002/j.1460-2075.1983.tb01661.x

Coding and 3' non-coding nucleotide sequence of chalcone synthase mRNA and assignment of amino acid sequence of the enzyme

Ursula Reimold 1, Manfred Kröger 1, Fritz Kreuzaler 1,1, Klaus Hahlbrock 1,1
PMCID: PMC555362  PMID: 16453477

Abstract

The nucleotide sequence of an almost complete cDNA copy of chalcone synthase mRNA from cultured parsley cells (Petroselinum hortense) has been determined. The cDNA copy comprised the complete coding sequence for chalcone synthase, a short A-rich stretch of the 5' non-coding region and the complete 3' non-coding region including a poly(A) tail. The amino acid sequence deduced from the nucleotide sequence of the cDNA is consistent with a partial N-terminal sequence analysis, the total amino acid composition, the cyanogen bromide cleavage pattern, and the apparent mol. wt. of the subunit of the purified enzyme.

Keywords: complementary DNA, nucleotide sequence, chalcone synthase, amino acid sequence, flavonoids

Full text

PDF
1801

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benoist C., O'Hare K., Breathnach R., Chambon P. The ovalbumin gene-sequence of putative control regions. Nucleic Acids Res. 1980 Jan 11;8(1):127–142. doi: 10.1093/nar/8.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Davies J. W., Aalbers A. M., Stuik E. J., Van Kammen A. Translation of cowpea mosaic virus RNA in a cell-free extract from wheat germ. FEBS Lett. 1977 May 15;77(2):265–269. doi: 10.1016/0014-5793(77)80248-2. [DOI] [PubMed] [Google Scholar]
  3. Dildrop R., Brüggemann M., Radbruch A., Rajewsky K., Beyreuther K. Immunoglobulin V region variants in hybridoma cells. II. Recombination between V genes. EMBO J. 1982;1(5):635–640. doi: 10.1002/j.1460-2075.1982.tb01220.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Grantham R., Gautier C., Gouy M. Codon frequencies in 119 individual genes confirm consistent choices of degenerate bases according to genome type. Nucleic Acids Res. 1980 May 10;8(9):1893–1912. doi: 10.1093/nar/8.9.1893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hahlbrock K., Knobloch K. H., Kreuzaler F., Potts J. R., Wellmann E. Coordinated induction and subsequent activity changes of two groups of metabolically interrelated enzymes. Light-induced synthesis of flavonoid glycosides in cell suspension cultures of Petroselinum hortense. Eur J Biochem. 1976 Jan 2;61(1):199–206. doi: 10.1111/j.1432-1033.1976.tb10012.x. [DOI] [PubMed] [Google Scholar]
  6. Heller W., Hahlbrock K. Highly purified "flavanone synthase" from parsley catalyzes the formation of naringenin chalcone. Arch Biochem Biophys. 1980 Apr 1;200(2):617–619. doi: 10.1016/0003-9861(80)90395-1. [DOI] [PubMed] [Google Scholar]
  7. Kozak M. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Res. 1981 Oct 24;9(20):5233–5252. doi: 10.1093/nar/9.20.5233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kreuzaler F., Ragg H., Fautz E., Kuhn D. N., Hahlbrock K. UV-induction of chalcone synthase mRNA in cell suspension cultures of Petroselinum hortense. Proc Natl Acad Sci U S A. 1983 May;80(9):2591–2593. doi: 10.1073/pnas.80.9.2591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kreuzaler F., Ragg H., Heller W., Tesch R., Witt I., Hammer D., Hahlbrock K. Flavanone synthase from Petroselinum hortense. Molecular weight, subunit composition, size of messenger RNA, and absence of pantetheinyl residue. Eur J Biochem. 1979 Aug 15;99(1):89–96. doi: 10.1111/j.1432-1033.1979.tb13235.x. [DOI] [PubMed] [Google Scholar]
  10. Kröger M., Kröger-Block A. A flexible new computer program for handling DNA sequence data. Nucleic Acids Res. 1982 Jan 11;10(1):229–236. doi: 10.1093/nar/10.1.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Langridge J., Langridge P., Bergquist P. L. Extraction of nucleic acids from agarose gels. Anal Biochem. 1980 Apr;103(2):264–271. doi: 10.1016/0003-2697(80)90266-3. [DOI] [PubMed] [Google Scholar]
  12. Lawton M. A., Dixon R. A., Hahlbrock K., Lamb C. J. Elicitor induction of mRNA activity. Rapid effects of elicitor on phenylalanine ammonia-lyase and chalcone synthase mRNA activities in bean cells. Eur J Biochem. 1983 Jan 17;130(1):131–139. [PubMed] [Google Scholar]
  13. Matern U., Heller W., Himmelspach K. Conformational changes of apigenin 7-O-(6-O-malonylglucoside), a vacuolar pigment from parsley, with solvent composition and proton concentration. Eur J Biochem. 1983 Jun 15;133(2):439–448. doi: 10.1111/j.1432-1033.1983.tb07483.x. [DOI] [PubMed] [Google Scholar]
  14. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  15. Palmiter R. D. Prevention of NH2-terminal acetylation of proteins synthesized in cell-free systems. J Biol Chem. 1977 Dec 25;252(24):8781–8783. [PubMed] [Google Scholar]
  16. Schröder J., Kreuzaler F., Schäfer E., Hahlbrock K. Concomitant induction of phenylalanine ammonia-lyase and flavanone synthase mRNAs in irradiated plant cells. J Biol Chem. 1979 Jan 10;254(1):57–65. [PubMed] [Google Scholar]
  17. Schüz R., Heller W., Hahlbrock K. Substrate specificity of chalcone synthase from Petroselinum hortense. Formation of phloroglucinol derivatives from aliphatic substrates. J Biol Chem. 1983 Jun 10;258(11):6730–6734. [PubMed] [Google Scholar]
  18. Sutcliffe J. G. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucleic Acids Res. 1978 Aug;5(8):2721–2728. doi: 10.1093/nar/5.8.2721. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES