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. 1983;2(10):1801–1805. doi: 10.1002/j.1460-2075.1983.tb01661.x

Coding and 3' non-coding nucleotide sequence of chalcone synthase mRNA and assignment of amino acid sequence of the enzyme

Ursula Reimold 1, Manfred Kröger 1, Fritz Kreuzaler 1,1, Klaus Hahlbrock 1,1
PMCID: PMC555362  PMID: 16453477

Abstract

The nucleotide sequence of an almost complete cDNA copy of chalcone synthase mRNA from cultured parsley cells (Petroselinum hortense) has been determined. The cDNA copy comprised the complete coding sequence for chalcone synthase, a short A-rich stretch of the 5' non-coding region and the complete 3' non-coding region including a poly(A) tail. The amino acid sequence deduced from the nucleotide sequence of the cDNA is consistent with a partial N-terminal sequence analysis, the total amino acid composition, the cyanogen bromide cleavage pattern, and the apparent mol. wt. of the subunit of the purified enzyme.

Keywords: complementary DNA, nucleotide sequence, chalcone synthase, amino acid sequence, flavonoids

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Selected References

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