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. 1984 Apr;3(4):873–877. doi: 10.1002/j.1460-2075.1984.tb01899.x

Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone.

P A Singer, W Lauer, Z Dembić, W E Mayer, J Lipp, N Koch, G Hämmerling, J Klein, B Dobberstein
PMCID: PMC557441  PMID: 6327293

Abstract

The invariant (Ii) chain is a membrane-spanning glycoprotein found intracellularly associated with class II major histocompatibility complex (MHC) molecules. Using hybrid-selected translation and the Ii-specific monoclonal antibody In-1, we have isolated a cDNA clone (pIi-5) coding for most of the Ii chain. Sequence analysis of this clone reveals an open reading frame encoding 169 amino acid residues. The protein is rich in methionine and contains two potential N-glycosylation sites. No stretch of uncharged amino acid residues, characteristic for a membrane-spanning segment, is found close to the COOH-terminal end. There is one, however, close to the NH2-terminal end. As it is know that approximately 20 amino acid residues of Ii chain are exposed on the cytoplasmic side, we conclude that the Ii chain spans the membrane exposing the NH2 terminus on the cytoplasmic side and the COOH terminus on the luminal side.

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Selected References

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