Photodynamic antifungal activity of a superparamagnetic and fluorescent drug carrier system against antibiotic-resistant fungal strains

Abstract

Increased antimicrobial resistance demands the development of new antimicrobial agents with high potency. A wide variety of microbial systems are continuously subjected to mutations which ultimately results in antimicrobial resistance. The present study aimed at the fabrication of a nano drug delivery system which simultaneously is superparamagnetic, fluorescent, non-cytotoxic and antifungal. The developed system is an easily targetable and detectable tool owing to its superparamagnetic and fluorescent characteristics respectively. Superparamagnetic iron oxide nanoparticles stabilized by macromolecular starch have been incorporated into a fluorescently modified carrier system to get the final drug delivery system. The finally developed drug carrier system is found to be non-cytotoxic from the in vitro cytotoxicity studies performed against normal rat spleen cells. The photodynamic antifungal capability of the system was the premier concern of investigation of the present study. The antifungal studies were conducted against Histoplasma capsulatum and Trichophyton rubrum by well diffusion method and the results were compared with the activity of the antibiotic, griseofulvin. The minimum inhibitory concentration against each fungal strain was determined using broth dilution method.

Graphic abstract

Supplementary Information

The online version contains supplementary material available at 10.1007/s10570-021-04107-y.

Introduction

Many decades past the discovery of antibiotics, microbial infections have again become a threat (Spellberg and Gilbert 2014). The antibiotic resistance crisis can be attributed to the overuse and misuse of these medications, as well as a lack of new drug development by the pharmaceutical industry due to reduced economic incentives and challenging regulatory requirements (Ventola 2015; Wright 2014; Viswanathan 2002; Read and Woods 2014). Even in 2020, the world is facing a crisis from a pandemic that has frozen the whole life on this planet. We have been shut down for months and living a new routine that this century has never experienced and all these due to a newly discovered corona virus. By this time we are all aware that these ultra-small species are most deadly to people with some other clinical history. Furthermore, more and more reports are coming on the post-covid bacterial and fungal infections caused by various microbial systems such as black fungus or mucor mould causing mucormycosis and yellow fungus causing mucor septicus etc. among the covid patients who survived covid 19 pandemic attack. Any microbe can turn dangerous at any point of time and we should be well equipped with medical tools which are at the same can fight these tiny species. These facts necessitate the need for more research in this field.

The biomedical research is busy with developing highly advanced therapeutic agents for treating fatal diseases. But all these efforts will be in vein if we leave the microbial infections untreated. In this context our study aimed at the development of a superparamagnetic, fluorescent, non-cytotoxic drug carrier which is at the same time antimicrobial.

Like other prominent metallic nanoparticles and their oxides (Ahmad et al. 2020; Shah et al. 2014), iron and iron oxide nanoparticles have already been recognized for their valid and crucial role as antimicrobial agents (Philip and Kuriakose 2017). We have synthesized superparamagnetic iron oxide nanoparticles by means of the macromolecular stabilizing system- starch. Ferrofluids containing encapsulated magnetic iron oxide nanoparticles (IONPs), can be employed for drug delivery (Jain et al. 2005; Chourpa et al. 2005; Dandamudi and Campbell 2007) to specific locations. Exploitation of superparamagnetic magnetization of iron oxide nanoparticles allows for “magnetic dragging” of internal nanoparticles (Blaney 2007)]. If the particle size measures higher than 200 nm, they are easily sequestered by the spleen and eventually removed by the phagocyte cells which results in reduced blood circulation times. Small particles whose size is less than 10 nm are easily removed by renal activities. In order to obtain a prolonged duration in the blood stream, the optimal size of iron oxide nanoparticles for intravenous injection should range in between 10–100 nm (Laurent et al. 2008). The incorporation of IONPs into starch in the present study aimed at the biocompatibility and superparamagnetism of the newly developed drug carrier.

The superparamagnetic iron oxide nanoparticles were then incorporated into a fluorescently modified starch conjugate. For the fluorescent modification of starch, 1-pyrene butyric acid (PBA) was used. PBA has been used in many labelling (Chen et al. 2011) and sensing (Chen et al. 2001) applications. The fluorescent modification enables easy identification of the developed system either in a living or a non-living body. The system was also analysed for its cytotoxic effect on normal rat spleen cells using Trypan blue dye exclusion assay.

The thematic focus of the photochromic/fluorescent modification discussed in this paper is the assessment of the developed system in photodynamic therapy. Photodynamic therapy (PDT) is coming up with new possibilities of treatment in medicine. It uses a photosensitizing drug (photosensitizer, PS) for the treatment of various diseases. When exposed to light with a particular wavelength, these drugs produce free oxygen radicals that can harm the nearby living cells (Kohanski et al. 2007). After excitation, the PS electrons at the singlet excited state will undergo intersystem crossing (ISC) to the triplet state. This triplet state can react with the biomolecules in two different ways (Fig. 1).

Fig. 1.

Mechanism of cell destruction in PDT

Type I reaction involves electron transfer from triplet state PS to an organic substrate within the cells, leading to the production of free radicals. These free radicals interact with oxygen in molecular level and produce reactive oxygen species (ROS) such as superoxide, hydroxyl radicals and hydrogen peroxide that can be harmful to the living cells. In Type II reaction, energy transfer occurs between the excited PS and the ground-state molecular oxygen, producing singlet oxygen that can interact with a large number of molecules in the cell to generate oxidized products (Huang et al. 2012; Foote 1991; Ghorbani et al. 2018).

PDT can be used in various fields of medicine inclusive of antimicrobial therapy, cancer treatment (Wang et al. 2002), ophthalmology and even for the treatment of acne. PDT can be used to effectively destroy microbes like bacteria, fungi and viruses (Rajan et al. 2016). When used for antimicrobial therapy it is more specifically termed as photodynamic antimicrobial chemotherapy (PACT). The finally developed superparamagnetic and fluorescent system was studied for its photodynamic antimicrobial potential against two highly pathogenic fungal strains- Histoplasma capsulatum and Trichophyton rubrum.

Histoplasma capsulatum lives in the environment, particularly in soil that contains large amounts of bird or bat droppings. They are usually found in the river valleys. It can cause histoplasmosis (Wheat et al. 2016) when people inhale these fungal spores from air. All those who breathe in the strain would not get infected. But the problem is serious when it comes with people with a weak immune system or those who are already sick.

Trichophyton rubrum is a dermatophytic fungus that colonizes the upper layers of dead skin, and is the most common cause of athlete’s foot (tinea pedis), fungal infection of nail, jock itch, and ringworm (Zaugg et al. 2009). T. rubrum infections are incredibly hard to diagnose, and difficult to differentiate from other dermatophytes since this fungal pathogen is poorly understood. Yet, these infections have been found to be susceptible to photodynamic treatment (Huang 2017).

Experimental

Materials

1-pyrene butyric acid (PBA, 97%), 4-dimethyl aminopyridine (DMAP, 99%), N,N′-dicyclohexyl carbodiimide (DCC, 99%), dimethyl formamide (DMF, 95%) and all other solvents were purchased from Sigma-Aldrich. Ferrous sulphate heptahydrate (FeSO₄.7H₂O, 99%) was purchased from Nice Chemicals Pvt. Ltd. India. Starch and sodium borohydride (NaBH₄, 98%) were purchased from Merck Specialities Pvt. Ltd. Mumbai. All the chemicals and reagents were used without any further purification. Double distilled water was used throughout the experiments.

Instrumentation

NMR spectra of the pure fluoroprobe and its modified analogues were recorded on a Bruker Avance NMR spectrometer (¹H, 500 MHz). Powder XRD studies were conducted using Rigaku Miniflex 600 XRD instrument operating at 1.54 Å with a CuKα source. A Jeol/JEM 2100 high resolution transmission electron microscope (HRTEM) operating at 200 kV was used to record the TEM images. The magnetic properties were analysed using a Lake Shore Cryotronics vibrating sample magnetometer (VSM). The UV/visible absorption spectrum was recorded on a Shimadzu UV/visible spectrophotometer in the range 190–1100 nm. Horiba Flouoromax-4- spectrofluorometer was used for fluorescence measurement.

Synthesis of starch stabilized, superparamagnetic iron oxide nanoparticles (IONP)

Fe²⁺ ions of the precursor FeSO₄.7H₂O were encapsulated into the macromolecular scaffolds of soluble starch by magnetic stirring. The encapsulated ferrous ions were then reduced to Fe(0) by sodium borohydride addition. The zero-valent iron was then air oxidized to Fe₂O₃. The detailed synthetic procedure is reported elsewhere (Philip and Kuriakose 2020).

Fluorescent modification of starch with 1-pyrene butyric acid by Steglich esterification (Neises and Steglich 1978)

To fluorescently modify macromolecular starch, the fluoroprobe 1-pyrene butyric acid (PBA) was used in molar excess as the study did not involve any quantitative estimation of the hydroxyl groups of starch. Starch, soluble (0.1 g) and pyrene butyric acid (0.5 g) in 10 ml DMF each were mixed together followed by addition of 10 ml DCC (0.5 g) in DMF. A catalytic amount of DMAP (200 mg) was added and the mixture was stirred at room temperature for 2 h and then at 80 °C for 6 h under nitrogen flushing. The mixture was then cooled to room temperature and then freezed at − 25 °C and filtered to remove the reaction byproduct dicyclohexyl urea (DCU) and all the unreacted molecules. The solvent was evaporated using a rotary flash evaporator and the product was dried. The dried fluorescent starch (FS) was then purified by column chromatography using 10:3 hexane–ethyl acetate mixture.

NMR spectra were recorded for pure PBA (Online Resource 1 Fig. S1) and the developed fluorescent starch-PBA aggregate (FS) (Online Resource 1 Fig. S2).

¹H NMR (PBA) (500 MHz, CDCl₃): δ 9.16 ppm (1H, s, –COOH proton ‘a’), δ 7.8–8.3 ppm (aromatic protons of pyrene), δ 3.44 ppm (2H, t, aliphatic protons ‘b’), δ 2.53 ppm (2H, t, aliphatic protons ‘d’), δ 2.25 ppm (2H, m, aliphatic protons ‘c’) and δ 7.3 ppm (CDCl₃ solvent).

¹H NMR (FS) (500 MHz, CDCl₃): δ 7.86–8.3 ppm (aromatic protons of pyrene), δ 3.41 ppm (2H, t, aliphatic protons ‘b’), δ 2.88 ppm (1H, s, COOR-CH- proton ester), δ 2.4 ppm (2H, t, aliphatic protons ‘d’), δ 2.23 ppm (2H, m, aliphatic protons ‘c’) and δ 7.26 ppm (CDCl₃ solvent).

The peak corresponding to –COOH proton around 9.16 ppm in PBA was absent in FS indicating the complete esterification of all the carboxylic groups of PBA with hydroxyl groups of starch. The formation of ester is further confirmed by a sharp peak at 2.88 ppm corresponding to α-hydrogens in starch bonded to –COOR group.

Development of the drug carrier (DC) system by doping superparamagnetic IONP into fluorescent starch-PBA conjugates (FS)

Fluorescently modified starch (FS) (0.010 g, 10 ml) and superparamagnetic IONP (0.010 g, 10 ml) dispersions in DMF were mixed thoroughly and magnetically stirred for 1 h at 80 °C and further for 4 h at 25 °C under nitrogen flushing. The solvent was evaporated using a rotary flash evaporator and the product was dried at room temperature in vacuum. The resultant drug carrier system, DC was purified using column chromatography with 8:2 (v/v) acetonitrile-methanol solvent system on a 100–200 mesh silica (Merck India Pvt. Ltd.) filled 2 × 20 cm adsorption column.

The synthetic procedures described for IONP, FS and DC are summarized respectively in the following Eqs. (1), (2) and (3) given below.

$${\text{Fe}}_2{\text{SO}}_4·7{\text{H}}_2\text{O}+\text{Starch}\underset{200{}^{\circ }\text{C}}{\overset{{\text{NaBH}}_4,{\text{O}}_2}{⟶}}\text{IONP (Superparamagnetic Iron Oxide Nanoparticle)}$$ 1

$$1-\text{Pyrene Butyric Acid (PBA)}+\text{Starch}\to \text{FS (Fluorescent Starch)}$$ 2

$$\text{IONP (from (1))}+\text{FS (from (2))}\to \text{DC (Drug Carrier)}$$ 3

In vitro cytotoxicity analysis against normal rat spleen cells

The test compound was studied for short term in vitro cytotoxicity using rat spleen cells. Rat was sacrificed using carbondioxide anaesthesia and the spleen tissue was dissected out. It was then smashed to single cell suspension in RPMI (Roswell Park Memorial Institute) complete medium containing antibiotics and filtered using mesh cloth. The collected cells were washed thrice and suspended in known volume of RPMI complete medium containing antibiotics and counted. Viable cell suspension (1 × 10⁶ cells in 0.1 ml) was added to tubes containing various concentrations of the test compound and the volume was made upto 1 ml using RPMI media. Control tubes contained only cell suspension (without additives). These tubes were mixed with 0.1 ml 1% trypan blue and kept for 2–3 min and loaded on a haemocytometer. Dead cells take up the blue colour of trypan blue while live cells do not take up the dye. The number of stained and unstained cells was counted separately using Eq. (4) given below.

$$\%cytoxicity=\frac{\mathit{Numberofdeadcells}}{Numberoflivecells+Numberofdeadcells}\times 100$$ 4

Agar well diffusion method for antifungal studies

Petriplates containing 20 ml Mueller Hinton medium were seeded with 24 h culture of fungal strains. Wells of diameter 6–8 mm were cut aseptically with a sterile cork borer into which 20 µl of the sample dispersion of desired concentration was introduced. The plates were then incubated at 37 °C for 24 h. The antifungal activity was assayed by measuring the diameter of the inhibition zone formed around the well.

Minimum inhibitory concentration (MIC): broth microdilution method

Minimum inhibitory concentration (MIC) of an antimicrobial agent is defined as its lowest concentration that can result in visible inhibition of the microbial growth. MIC also establishes the level of microbial resistance to an antimicrobial agent. The minimum inhibitory concentration (MIC) was determined using broth micro dilution method. 50 μl of sample solution in twofold dilutions (2.5 μg/ml, 5 μg/ml, 10 μg/ml, 20 μg/ml and 40 μg/ml) were prepared in broth medium using a 96-well microtitration plate. Each well is then inoculated with 50 μl of the microbial inoculum prepared in the same growth medium after dilution to 0.5 McFarland scale. After well-mixing, the inoculated wells were incubated for 18 h at 37 °C. MIC was taken as the lowest concentration that causes a visible inhibition of fungal growth which is indicated by absence of turbidity on addition of the antimicrobial solution. CLSI has standardized the broth dilution protocol for fungi.

Results and discussions

The superparamagnetic IONP and the IONP encapsulated drug carrier systems were characterized by different analytical techniques and then undergone for PDT studies.

XRD studies

Powder XRD was performed on the superparamagnetic IONP and the final drug carrier system, DC into which IONP has been doped. Both the samples showed distinct diffraction pattern (Fig. 2) characteristic of α-Fe₂O₃ nanoparticles (JCPDS No. 33-0664). No other phases of iron oxide were present as impurities. The retention of well-defined peaks of crystalline α-Fe₂O₃ nanoparticles even after doping is a sign of enhanced stability of the nanoparticles within the macromolecular scaffolds of the stabilizing system-starch.

Fig. 2.

XRD pattern of a IONP and b IONP incorporated drug carrier DC

Debye Scherrer equation, $d=K\lambda /\beta cos\mathrm{\Theta }.$ was used to calculate the particle size. IONPs before doping were found to be in the particle size range of 12–17 nm. The particle size was calculated to be in the range of 12–24 nm for IONPs after being doped to get the drug carrier. This mild but negligible increase in particle size may be due to possibility for slight aggregation.

HRTEM analysis

High resolution TEM images were recorded for the samples IONP and DC (Fig. 3). IONPs before doping (Fig. 3a) exhibit distinct hexagonal iron oxide nanoparticles which again confirms the characteristics of α-Fe₂O₃. The particles more or less fall around 20 nm in particle size which is almost in agreement with the XRD results.

Fig. 3.

HRTEM images of nanoparticles in a IONP and b IONP incorporated drug carrier Drug carrier (DC)

Figure 3b reveals the microscopic morphology of the finally developed drug carrier (DC), which has been synthesized by incorporationg superparamagnetic IONPs into the fluorescent starch (FS). The image reveals particles those exhibit a slight degree of aggregation which resulted in slight particle size increase as predicted by XRD. Apart from the mild degree of aggregation, the successful incorporation of IONPs into the drug delivery system is confirmed by XRD and HRTEM analyses.

Magnetic properties

The magnetic properties of the systems—IONP and DC were studied using a vibrating sample magnetometer (VSM). The measurements were done at room temperature (Fig. 4).

Fig. 4.

M–H curve of a IONP and b IONP incorporated drug carrier (DC)

The two samples exhibited M–H curves without the evidence of any hysteresis which is an indication of their superparamagnetic behaviour. The coercivity of the sample was observed to be increased to 69.860 G from 20.371 G upon incorporation into the fluorescent system which may be due to the aggregation of particles. However the retention of superparamagnetic behaviour of IONPs even after doping into the fluorescently modified macromolecular scaffolds is promising while their drug carrier potential is to be considered. The superparamagnetic particles can easily be targeted to the site of action by an external magnetic field. This précised targeting can, of course, reduce the complications of a treatment protocol.

UV/visible absorption and Photoluminescence spectra

The UV/visible absorption spectrum of the IONP incorporated drug carrier system (DC) was recorded in DMF in the range 190–1100 nm (Fig. 5a). Three characteristic absorption peaks of pyrenyl moiety were observed above 300 nm. Photoluminescence (PL) emission spectrum of DC was recorded at an excitation wavelength of 295 nm (Fig. 5b). The excitation wavelength was fixed at 295 nm owing to the well characterized absorptions of pyrenyl species above 300 nm.

Fig. 5.

a UV/visible absorption and b Photoluminescence spectra of the drug carrier (DC)

The first three intense peaks—378 nm, 398 nm and 420 nm are characteristic emissions of pyrene monomers from PBA used for the fluorescent modification of starch and the weak peak broad one around 445 nm indicates a negligible amount of pyrene as dimers in the excited state (excimers) (Philip and Kuriakose 2019). The spectrum confirms the successful esterification between PBA and starch. The emission of the drug carrier solution in UV light is given in the inset of Fig. 5b, which clearly reveals the fluorescent nature of the newly developed drug delivery system.

In vitro cytotoxicity analysis of the superparamagnetic fluorescent drug carrier (DC)

The short term in vitro cytotoxicity analysis was conducted for the finally developed drug carrier, DC at five different concentrations in DMF (10 μg/ml, 20 μg/ml, 50 μg/ml, 100 μg/ml and 200 μg/ml) against normal rat spleen cells and the results are noted in Table 1. The graphical representation is given in Fig. 6. Linear trendline is plotted which can be used to calculate the IC₅₀ (half maximal inhibitory concentration) value by regression analysis. IC₅₀ is a measure of the potency of a substance or drug in inhibiting a specific biological or biochemical function. It is the concentration of a drug that is required for 50% inhibition of a biological process or component in vitro.

Table 1.

In vitro cytotoxicity analysis of DC against normal rat spleen cells

Cell line	Percentage cell death at					Control (cell death)	IC50 (mg/ml)
	10 μg/ml	20 μg/ml	50 μg/ml	100 μg/ml	200 μg/ml
Normal	0	0	0	2	4	4%	2.30

Fig. 6.

Linear regression analysis for the cytotoxicity analysis of DC against normal rat spleen cells

It is clear from Table 1 that our system exhibited only the least cell death of 4% even at the maximum concentration of 200 μg/ml. It is worth to mention that even the control cell death was 4%. The results have proven the non-cytotoxicity of the system.

Photodynamic antifungal activity and MIC determination of the newly developed superparamagnetic, fluorescent and non-cytotoxic drug carrier system

Antifungal activity of pure fluoroprobe (PBA), functionally modified starch (FS) and the final drug carrier (DC) against H. capsulatum and T. Rubrum was studied by well diffusion method. PDT (photodynamic therapy) studies of the drug carrier were conducted after irradiating DC for 2 h using a Newport Xenon arc lamp of 300 W. The time for irradiation was optimized to be 2 h as there was no observed increase in the antimicrobial activity after 2 h. The sample after irradiation is denoted as DC*. The activities of the samples were compared with the antibiotic, griseofulvin. Water is the negative control. The results are shown in Fig. 7 and zones of inhibition are noted in Table 2.

Fig. 7.

Photodynamic antifungal activity against a H. capsulatum and b T. rubrum (‘*’: irradiated sample)

Table 2.

Antifungal zones of inhibition in PACT

Fungal strain/sample	Zone of inhibition ± standard deviation (mm)
	PBA	FS	DC	DC*	Griseofulvin (antibiotic)
H. capsulatum	0	27.00 ± 1.67	44.00 ± 0.89	54.17 ± 1.72a	0
T. rubrum	0	20.17 ± 0.98	45.00 ± 1.55	54.33 ± 0.82a	0
P ≤ 0.05 F = 435.949

‘*’Irradiated sample. The significance level ‘a’ predicted by DMRT says that the two results are statistically equivalent

Minimum inhibitory concentration (MIC) against each strain was determined for the irradiated sample (DC*) using micro broth dilution method. MIC is the minimum concentration of an antimicrobial that visibly inhibits the growth of the microbe. The experiments were performed at five different concentrations—2.5 μg/ml, 5 μg/ml, 10 μg/ml, 20 μg/ml and 40 μg/ml to determine the MIC.

All the measurements were done in six replicates and one-way ANOVA was carried out on all the results. Duncan’s multiple range test (DMRT) is used as the post-hoc test at a significance level of p ≤ 0.05 using SPSS 16.0 software in the present statistics.

The fluoroprobe PBA was found to be inactive against the two strains in the study. FS and DC exhibited considerable activity against the two microbes and their zones of inhibition were almost similar against each strain. But the point of interest is the activity boost observed upon irradiation of the sample. The irradiated sample, DC* have marked an increase in zone diameter of about 10 mm against each strain. The above mentioned result is an indication of the success of our presently modified drug system in photodynamic therapy (PDT) or more specifically photodynamic antimicrobial chemotherapy (PACT). The irradiated system showed remarked activity against T.rubrum (54.33 ± 0.82 mm) and H.capsulatum (54.17 ± 1.72 mm). Another important finding is that these strains found highly susceptible in our PDT studies are found to be resistant to the currently used antibiotic, griseofulvin. Griseofulvin had no zone of inhibition against any of the two strains.

Membrane disruption and cell cycle arrest are the most proposed mechanisms of the antifungal activity of the antimicrobials involving nanoparticles (Kumari et al. 2019; Kim et al. 2009). Reactive oxygen species (ROS) dependent fungal cell death can also be a reason (Das et al. 2016). ROS could be the predominant mechanism of fungal inhibition in PDT. Highly reactive species generated from excited PS may harm the microbial cells by type-I or type-II inhibition as detailed in introduction.

The graphical analysis is given in Fig. 8 in which the increase in antifungal activity upon irradiation is clearly established. MIC values for each strain is determined and noted in Table 3. MIC value determined for each strain in PDT is 2.5 μg/ml. The ‘-’ sign against each concentration in table 3 denotes the inhibition of microbial growth at the particular concentration. 

Fig. 8.

Graphical plot of photodynamic antifungal activity 20 μg/ml

Table 3.

Antifungal zones of inhibition and MIC values of DC* in PACT (Photodynamic antimicrobial chemotherapy)

Fungal strain	Zone of inhibition ± standard deviation (mm)	MIC determination					MIC (μg/ml)
		2.5 μg/ml	5 μg/ml	10 μg/ml	20 μg/ml	40 μg/ml
H. capsulatum	54.17 ± 1.72	–	–	–	–	–	2.5
T. rubrum	54.33 ± 0.82	–	–	–	–	–	2.5

Conclusions

The instant biomimicing capacity of infectious microbes leads to withdrawal of many novel antibiotics within a short period. Increased prevalence in antimicrobial resistance to traditional antibiotics necessitates the need for development of novel and much more improved antimicrobial agents. The present paper discussed the development of a novel antimicrobial agent with potent photodynamic activity. The system besides its antifungal property possesses superparamagnetic and fluorescent characteristics with absolutely non-cytotoxic traits. The use of starch for the stabilization of nanoparticles as well as for the construction of the drug carrier renders the system more biocompatible. The drug carrier system developed can be precisely targeted due to its superparamagnetism and easily detected owing to its fluorescence. The presently developed system deserves a special attention with regard to its photodynamic antifungal activity against antibiotic-resistant strains and the results sound promising with zones of inhibition around 55 mm. The MIC against two strains was determined to be 2.5 μg/ml in PDT and the system is non-toxic upto 200 μg/ml. The result is an assurance of the system to be developed into a multi-tasking drug delivery system with far reaching potentials.

Supplementary Information

Below is the link to the electronic supplementary material.

Authors’ contributions

Both the authors contributed to the study conception and design. Synthesis and analyses were performed by the first author Sherin Philip. The first draft of the manuscript was prepared by the first author and commented and modified by the corresponding author Sunny Kuriakose. Both the authors read and approved the final manuscript.

Funding

Current research was funded by University Grants Commission (India) under the Senior Research Fellowship scheme (Sr.No-2061410045 Ref. No-22/06/2014 (i) EU-V).

Availability of data and materials

Not applicable.

Declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethics approval

The authors declare that the study conducted meet all the ethical standards as specified by the journal and did not involve any animal/human research. The data and materials as well as software applications used in the study comply with field standards.