Files in this data supplement:
- Supporting Information: Figures S1-S8 and Tables S1-S5 (PDF, 2 MB)
- Figure S1: Linkage and physical maps of Qphs.pseru-3AS (PDF, 342 KB)
- Figure S2: Markers used to determine the chromosome location of Qphs.pseru-3AS (PDF, 326 KB)
- Figure S3: BAC contig Ctg619 (bottom solid bar) spans the entire Qphs.pseru-3AS region. (PDF, 253 KB)
- Figure S4: Sequence alignment of the deduced amino acids of TaPHS1 homologs from maize (GRMZM2G021614, GRMZM2G152689, GRMZM2G059358), sorghum (Sb03g8270), Brachypodium (Bradi2g01020), barley (HvMFT), rice (Os01g02120, Os06g30370), Arabidopsis (At01g18100), and wheat (Qphs.pseru-3AS). (PDF, 621 KB)
- Figure S5: Comparison of the promoter sequences between Rio Blanco and NW97S186 (PDF, 286 KB)
- Figure S6: Linkage disequilibrium (LD) between the two causal SNPs (+646 and +666) in intron 3 and 18 polymorphic sites of TaPHS1 analyzed in 82 wheat cultivars. (PDF, 528 KB)
- Figure S7: Two causal SNPs determine PHS resistance (PDF, 236 KB)
- Figure S8: One diagnostic SNP markers for TaPHS1 (TaPHS1-SNP1) was developed based on the GT-to-AT mutation at the mis-splicing site (+646) in susceptible cultivars. (PDF, 363 KB)
- Table S1: Primers used for sequencing, gene expression, and gene transformation (PDF, 229 KB)
- Table S2: Genotypic data and sprouting resistance levels of the 56 recombinants analyzed with the markers in the QTL Qphs.pseru-3AS region (PDF, 288 KB)
- Table S3: Thirteen genes that were mapped in the QTL Qphs.pseru-3AS region by comparative mapping (PDF, 277 KB)
- Table S4: Genes identified by sequencing the three BACs spanning the Qphs.pseru-3AS region (PDF, 273 KB)
- Table S5: Relationship among two structure groups, seed color, and causal SNPs for PHS resistance in TaPHS1 (PDF, 294 KB)