Genetic and pharmacological inhibition of acid ceramidase prevents asymmetric cell division by neosis

Supplemental Data

• Supplemental Figure S1 (.pptx, 74 KB) - 8 Gy radiation bolus treatment has the same effect as fractionated 8 Gy.
• Supplemental Figure S2 (.pptx, 100 KB) - PGCC are characterized by high propidium iodide and high SCC/FSC by flow cytometry.
• Supplemental Figure S3 (.pptx, 61 KB) - DNA accumulation does not lead to a concomitant rise in RNA expression.
• Supplemental Figure S4 (.pptx, 245 KB) - Inhibition of ASAH1 via shRNA targeting or LCL-521 results in accumulation of cellular ceramide.
• Supplemental Figure S5 (.pptx, 1.1 MB) - A549 NSCLC cells express ASAH1, accumulate ceramide upon LCL-521 exposure, and are capable of neosis after treatment stress.
• Supplemental Figure S8 (.pptx, 70 KB) - Inhibition of neosis by targeting ASAH1 using LCL-521, tamoxifen or carmofur.