Skip to main content
PMC home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1990 Sep;10(9):4957–4960. doi: 10.1128/mcb.10.9.4957

Highly repeated sites in the apolipoprotein(a) gene recognized by methylated DNA-binding protein, a sequence-specific DNA-binding protein.

K C Ehrlich 1, M Ehrlich 1
PMCID: PMC361120  PMID: 2388631

Abstract

Methylated DNA-binding protein (MDBP), a sequence-specific DNA-binding protein, was found to recognize more than 30 sites within an allele of the human apolipoprotein(a) gene. High plasma levels of apolipoprotein(a), a risk factor for atherosclerosis, have been correlated with genetically inherited lower-molecular-mass isoforms of this protein. MDBP might help down modulate the expression of the apolipoprotein(a) gene in a manner dependent on the length of a given allele of the gene and the number of MDBP sites in it.

Full text

PDF
4958

Images in this article

4958Image
on p.4958
4958Image
on p.4958

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Drayna D. T., Hegele R. A., Hass P. E., Emi M., Wu L. L., Eaton D. L., Lawn R. M., Williams R. R., White R. L., Lalouel J. M. Genetic linkage between lipoprotein(a) phenotype and a DNA polymorphism in the plasminogen gene. Genomics. 1988 Oct;3(3):230–236. doi: 10.1016/0888-7543(88)90084-5. [DOI] [PubMed] [Google Scholar]
  2. Ehrlich M., Wang R. Y. 5-Methylcytosine in eukaryotic DNA. Science. 1981 Jun 19;212(4501):1350–1357. doi: 10.1126/science.6262918. [DOI] [PubMed] [Google Scholar]
  3. Forsgren M., Råden B., Israelsson M., Larsson K., Hedén L. O. Molecular cloning and characterization of a full-length cDNA clone for human plasminogen. FEBS Lett. 1987 Mar 23;213(2):254–260. doi: 10.1016/0014-5793(87)81501-6. [DOI] [PubMed] [Google Scholar]
  4. Gama-Sosa M. A., Midgett R. M., Slagel V. A., Githens S., Kuo K. C., Gehrke C. W., Ehrlich M. Tissue-specific differences in DNA methylation in various mammals. Biochim Biophys Acta. 1983 Jun 24;740(2):212–219. doi: 10.1016/0167-4781(83)90079-9. [DOI] [PubMed] [Google Scholar]
  5. Glass C. K., Holloway J. M., Devary O. V., Rosenfeld M. G. The thyroid hormone receptor binds with opposite transcriptional effects to a common sequence motif in thyroid hormone and estrogen response elements. Cell. 1988 Jul 29;54(3):313–323. doi: 10.1016/0092-8674(88)90194-8. [DOI] [PubMed] [Google Scholar]
  6. Hajjar K. A., Gavish D., Breslow J. L., Nachman R. L. Lipoprotein(a) modulation of endothelial cell surface fibrinolysis and its potential role in atherosclerosis. Nature. 1989 May 25;339(6222):303–305. doi: 10.1038/339303a0. [DOI] [PubMed] [Google Scholar]
  7. Hixson J. E., Britten M. L., Manis G. S., Rainwater D. L. Apolipoprotein(a) (Apo(a)) glycoprotein isoforms result from size differences in Apo(a) mRNA in baboons. J Biol Chem. 1989 Apr 15;264(11):6013–6016. [PubMed] [Google Scholar]
  8. Huang L. H., Wang R., Gama-Sosa M. A., Shenoy S., Ehrlich M. A protein from human placental nuclei binds preferentially to 5-methylcytosine-rich DNA. Nature. 1984 Mar 15;308(5956):293–295. doi: 10.1038/308293a0. [DOI] [PubMed] [Google Scholar]
  9. Hyman S. E., Comb M., Pearlberg J., Goodman H. M. An AP-2 element acts synergistically with the cyclic AMP- and phorbol ester-inducible enhancer of the human proenkephalin gene. Mol Cell Biol. 1989 Jan;9(1):321–324. doi: 10.1128/mcb.9.1.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Imagawa M., Chiu R., Karin M. Transcription factor AP-2 mediates induction by two different signal-transduction pathways: protein kinase C and cAMP. Cell. 1987 Oct 23;51(2):251–260. doi: 10.1016/0092-8674(87)90152-8. [DOI] [PubMed] [Google Scholar]
  11. Khan R., Zhang X. Y., Supakar P. C., Ehrlich K. C., Ehrlich M. Human methylated DNA-binding protein. Determinants of a pBR322 recognition site. J Biol Chem. 1988 Oct 5;263(28):14374–14383. [PubMed] [Google Scholar]
  12. Koschinsky M. L., Beisiegel U., Henne-Bruns D., Eaton D. L., Lawn R. M. Apolipoprotein(a) size heterogeneity is related to variable number of repeat sequences in its mRNA. Biochemistry. 1990 Jan 23;29(3):640–644. doi: 10.1021/bi00455a007. [DOI] [PubMed] [Google Scholar]
  13. Kratzin H., Armstrong V. W., Niehaus M., Hilschmann N., Seidel D. Structural relationship of an apolipoprotein (a) phenotype (570 kDa) to plasminogen: homologous kringle domains are linked by carbohydrate-rich regions. Biol Chem Hoppe Seyler. 1987 Dec;368(12):1533–1544. doi: 10.1515/bchm3.1987.368.2.1533. [DOI] [PubMed] [Google Scholar]
  14. Lindahl G., Gersdorf E., Menzel H. J., Duba C., Cleve H., Humphries S., Utermann G. The gene for the Lp(a)-specific glycoprotein is closely linked to the gene for plasminogen on chromosome 6. Hum Genet. 1989 Jan;81(2):149–152. doi: 10.1007/BF00293891. [DOI] [PubMed] [Google Scholar]
  15. Malinowski D. P., Sadler J. E., Davie E. W. Characterization of a complementary deoxyribonucleic acid coding for human and bovine plasminogen. Biochemistry. 1984 Aug 28;23(18):4243–4250. doi: 10.1021/bi00313a035. [DOI] [PubMed] [Google Scholar]
  16. McLean J. W., Tomlinson J. E., Kuang W. J., Eaton D. L., Chen E. Y., Fless G. M., Scanu A. M., Lawn R. M. cDNA sequence of human apolipoprotein(a) is homologous to plasminogen. Nature. 1987 Nov 12;330(6144):132–137. doi: 10.1038/330132a0. [DOI] [PubMed] [Google Scholar]
  17. Miles L. A., Fless G. M., Levin E. G., Scanu A. M., Plow E. F. A potential basis for the thrombotic risks associated with lipoprotein(a). Nature. 1989 May 25;339(6222):301–303. doi: 10.1038/339301a0. [DOI] [PubMed] [Google Scholar]
  18. Mitchell P. J., Wang C., Tjian R. Positive and negative regulation of transcription in vitro: enhancer-binding protein AP-2 is inhibited by SV40 T antigen. Cell. 1987 Sep 11;50(6):847–861. doi: 10.1016/0092-8674(87)90512-5. [DOI] [PubMed] [Google Scholar]
  19. Shore D., Nasmyth K. Purification and cloning of a DNA binding protein from yeast that binds to both silencer and activator elements. Cell. 1987 Dec 4;51(5):721–732. doi: 10.1016/0092-8674(87)90095-x. [DOI] [PubMed] [Google Scholar]
  20. Supakar P. C., Weist D., Zhang D. L., Inamdar N., Zhang X. Y., Khan R., Ehrlich K. C., Ehrlich M. Methylated DNA-binding protein is present in various mammalian cell types. Nucleic Acids Res. 1988 Aug 25;16(16):8029–8044. doi: 10.1093/nar/16.16.8029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Supakar P. C., Zhang X. Y., Githens S., Khan R., Ehrlich K. C., Ehrlich M. How different DNA sequences are recognized by a DNA-binding protein: effects of partial proteolysis. Nucleic Acids Res. 1989 Nov 11;17(21):8611–8629. doi: 10.1093/nar/17.21.8611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Thierry F., Yaniv M. The BPV1-E2 trans-acting protein can be either an activator or a repressor of the HPV18 regulatory region. EMBO J. 1987 Nov;6(11):3391–3397. doi: 10.1002/j.1460-2075.1987.tb02662.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tomlinson J. E., McLean J. W., Lawn R. M. Rhesus monkey apolipoprotein(a). Sequence, evolution, and sites of synthesis. J Biol Chem. 1989 Apr 5;264(10):5957–5965. [PubMed] [Google Scholar]
  24. Utermann G. The mysteries of lipoprotein(a). Science. 1989 Nov 17;246(4932):904–910. doi: 10.1126/science.2530631. [DOI] [PubMed] [Google Scholar]
  25. Zhang D. L., Ehrlich K. C., Supakar P. C., Ehrlich M. A plant DNA-binding protein that recognizes 5-methylcytosine residues. Mol Cell Biol. 1989 Mar;9(3):1351–1356. doi: 10.1128/mcb.9.3.1351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Zhang X. Y., Supakar P. C., Khan R., Ehrlich K. C., Ehrlich M. Related sites in human and herpesvirus DNA recognized by methylated DNA-binding protein from human placenta. Nucleic Acids Res. 1989 Feb 25;17(4):1459–1474. doi: 10.1093/nar/17.4.1459. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES