Abstract
Toluidine blue staining was used to detect Chlamydia trachomatis inclusions in both McCoy and HeLa-229 cells from clinical specimens. This method was more sensitive than iodine staining for detecting C trachomatis inclusions in both McCoy and HeLa-229 cells and also more sensitive than Giemsa staining for detecting chlamydial inclusions in HeLa-229 cells. While its sensitivity for detection of chlamydial inclusions in McCoy cells is equal to that of Giemsa staining, toluidine blue staining is easier and faster to perform.
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- Croy T. R., Kuo C. C., Wang S. P. Comparative susceptibility of eleven mammalian cell lines to infection with trachoma organisms. J Clin Microbiol. 1975 May;1(5):434–439. doi: 10.1128/jcm.1.5.434-439.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davies J. A., Rees E., Hobson D., Karayiannis P. Isolation of Chlamydia trachomatis from Bartholin's ducts. Br J Vener Dis. 1978 Dec;54(6):409–413. doi: 10.1136/sti.54.6.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans R. T., Woodland R. M. Detection of chlamydiae by isolation and direct examination. Br Med Bull. 1983 Apr;39(2):181–186. doi: 10.1093/oxfordjournals.bmb.a071813. [DOI] [PubMed] [Google Scholar]
- GORDON F. B., QUAN A. L. OCCURENCE OF GLYCOGEN IN INCLUSIONS OF THE PSITTACOSIS-LYMPHOGRANULOMA VENEREUM-TRACHOMA AGENTS. J Infect Dis. 1965 Apr;115:186–196. doi: 10.1093/infdis/115.2.186. [DOI] [PubMed] [Google Scholar]
- Johnson F. W. A comparison of staining techniques for demonstrating group A chlamydia in tissue culture. Med Lab Technol. 1975 Jul;32(3):233–238. [PubMed] [Google Scholar]
- Johnson F. W., Chancerelle L. Y., Hobson D. An improved method for demonstrating the growth of Chlamydiae in tissue culture. Med Lab Sci. 1978 Jan;35(1):67–74. [PubMed] [Google Scholar]
- Johnson F. W., Hobson D. Factors affecting the sensitivity of replicating McCoy cells in the isolation and growth of chlamydia A (TRIC agents). J Hyg (Lond) 1976 Jun;76(3):441–451. doi: 10.1017/s0022172400055376. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mallinson H., Sikotra S., Arya O. P. Cultural method for large-scale screening for Chlamydia trachomatis genital infection. J Clin Pathol. 1981 Jul;34(7):712–718. doi: 10.1136/jcp.34.7.712. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woodland R. M., El-Sheikh H., Darougar S., Squires S. Sensitivity of immunoperoxidase and immunofluorescence staining for detecting chlamydia in conjunctival scrapings and in cell culture. J Clin Pathol. 1978 Nov;31(11):1073–1077. doi: 10.1136/jcp.31.11.1073. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woodland R. M., Malam J., Darougar S. A rapid method for staining inclusions of Chlamydia psittaci and Chlamydia trachomatis. J Clin Pathol. 1982 Jun;35(6):642–644. doi: 10.1136/jcp.35.6.642. [DOI] [PMC free article] [PubMed] [Google Scholar]