Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200712036
Cao et al.

• Figure S1 GFP-Moesin marks F-actin cap and furrow.

• Figure S2 Jasp induces excess actin polymerization around the furrow.

• Figure S3 LatA injection results in disruption of GFP-Dlg and Spider-GFP–marked furrow plasma membrane integrity.

• Figure S4 Suppression of nuf phenotypes.

• Figure S5 Localization of RhoGEF2 and F-actin in nuf and Rab11 embryos.

• Video 1 F-actin signal is rapidly recovered at the furrow after photobleaching.

• Video 2 LatA injection results in loss of membrane integrity and spindle fusions.

• Video 3 F-actin is not stably maintained in nuf embryos.

• Video 4 F-actin loss precedes membrane loss in nuf embryos.

• Video 5 Reducing the dosage of Twinstar stabilizes F-actin at the furrow in nuf embryos.

• Video 6 GFP-Dlg–marked furrow membrane is not stably maintained in nuf embryos treated with DMSO.

• Video 7 Jasp injection stabilizes membrane at the furrow in nuf embryos.

• Video 8 C3 exotransferase injection results in loss of membrane integrity and spindle fusions.

• Video 9 RhoA(Q63L) suppresses the nuf furrow defects local to the injection site.