ABSTRACT
Bacterial biofilm communities are associated with profound physiological changes that lead to novel properties compared to the properties of individual (planktonic) bacteria. The study of biofilm-associated phenotypes is an essential step toward control of deleterious effects of pathogenic biofilms. Here we investigated lipopolysaccharide (LPS) structural modifications in Escherichia coli biofilm bacteria, and we showed that all tested commensal and pathogenic E. coli biofilm bacteria display LPS modifications corresponding to an increased level of incorporation of palmitate acyl chain (palmitoylation) into lipid A compared to planktonic bacteria. Genetic analysis showed that lipid A palmitoylation in biofilms is mediated by the PagP enzyme, which is regulated by the histone-like protein repressor H-NS and the SlyA regulator. While lipid A palmitoylation does not influence bacterial adhesion, it weakens inflammatory response and enhances resistance to some antimicrobial peptides. Moreover, we showed that lipid A palmitoylation increases in vivo survival of biofilm bacteria in a clinically relevant model of catheter infection, potentially contributing to biofilm tolerance to host immune defenses. The widespread occurrence of increased lipid A palmitoylation in biofilms formed by all tested bacteria suggests that it constitutes a new biofilm-associated phenotype in Gram-negative bacteria.
IMPORTANCE
Bacterial communities called biofilms display characteristic properties compared to isolated (planktonic) bacteria, suggesting that some molecules could be more particularly produced under biofilm conditions. We investigated biofilm-associated modifications occurring in the lipopolysaccharide (LPS), a major component of all Gram-negative bacterial outer membrane. We showed that all tested commensal and pathogenic biofilm bacteria display high incorporation of a palmitate acyl chain into the lipid A part of LPS. This lipid A palmitoylation is mediated by the PagP enzyme, whose expression in biofilm is controlled by the regulatory proteins H-NS and SlyA. We also showed that lipid A palmitoylation in biofilm bacteria reduces host inflammatory response and enhances their survival in an animal model of biofilm infections. While these results provide new insights into the biofilm lifestyle, they also suggest that the level of lipid A palmitoylation could be used as an indicator to monitor the development of biofilm infections on medical surfaces.
INTRODUCTION
Biofilm communities developing on medical and industrial surfaces constitute a recognized reservoir of bacterial pathogens, and control of biofilm-associated infections is a major focus of microbiology at this time (1, 2). Identification of key biofilm determinants in several pathogens led to potential antibiofilm strategies based either on prevention of initial bacterial adhesion, inhibition of biofilm maturation, biofilm dispersion, or eradication of highly antibiotic-tolerant biofilm bacteria (3, 4). Study of gene expression and physiological changes occurring during biofilm formation suggested that specific molecules might be associated with the biofilm environment (5–8). Consistently, several compounds were shown to accumulate within biofilms, including biofilm matrix components, antiadhesion molecules, and amino acids (9–11).
We previously showed that formation of biofilms by certain Pseudomonas aeruginosa strains induced reversible loss of lipopolysaccharide (LPS) O antigen and alteration of lipid A that contributes to modulating the host inflammatory response to P. aeruginosa biofilms (12). LPS is a major component of all Gram-negative bacterial outer membranes, and although its structure varies in response to certain environmental stimuli (13), few studies have investigated modifications in LPS structure in biofilms (12, 14, 15). Here, we compared Escherichia coli LPS from biofilm and planktonic bacteria and identified a reversible increased LPS modification corresponding to incorporation of a palmitate acyl chain to lipid A (palmitoylation) mediated by the PagP enzyme. While the appearance of this LPS modification is correlated to the ability of the bacteria to form biofilms, it occurs progressively and only after several days in very long stationary-phase culture bacteria. We show that pagP is negatively regulated by H-NS, which itself is under the biofilm-regulated control of the SlyA regulator. We demonstrate that increased lipid A palmitoylation in biofilm bacteria decreases the inflammatory response and increases biofilm bacterial survival in an in vivo rat model of catheter-associated biofilm infection. Since increased lipid A palmitoylation also occurs in biofilms formed by a wide range of Gram-negative bacteria, our study therefore identified a new biofilm phenotype, which could be used as a biofilm biomarker to monitor Gram-negative bacterial biofilm-associated infections.
RESULTS
Evidence for biofilm-associated LPS modification in E. coli biofilm.
To identify potential modifications of LPS in E. coli biofilms, we compared patterns of rough LPS (Ra, with no O antigen) produced in biofilm and planktonic E. coli K-12 BW25113 bacteria. Tricine SDS-PAGE analysis revealed that LPS extracted from 96-h mature biofilm grown in microfermentors with constant medium renewal had a higher molecular weight than LPS species extracted from 15- to 24-h overnight stationary-phase planktonic culture (Fig. 1A; see Fig. S1 in the supplemental material). An additional band was progressively detected along with aging biofilms and disappeared in LPS extracted from 96-h biofilm bacteria reinoculated under planktonic conditions (Fig. 1A). We also observed an additional LPS band associated with biofilms formed by various pathogenic E. coli strains (Fig. 1B). To further investigate the correlation between biofilm formation and appearance of the additional LPS band, we compared the E. coli BW25113 strain with its closely related derivative, the BW25113 F strain, forming more biofilm than BW25113 due to the presence of the biofilm-promoting F conjugative plasmid (Fig. 1C) (16). Analysis of LPS extracted from biofilms formed by E. coli BW25113 and BW25113 F strains at different time points showed that the additional LPS band appeared more rapidly in the BW25113 F strain (48 h versus 72 h) (Fig. 1D). Whereas this suggested a link between biofilm capacity and LPS modification, monitoring LPS profiles in planktonic cultures (without medium renewal) also revealed the emergence of the additional LPS band in aging planktonic culture (48 h and beyond) (see Fig. S2A in the supplemental material). In contrast, the regular renewal of the growth medium in these very late stationary-phase cultures, performed to limit nutrient exhaustion and approximate microfermentor conditions, significantly reduced the appearance of this band (Fig. S2B). These results therefore suggested that the studied LPS modification occurs in mature biofilms and under conditions created in unusually long planktonic cultures.
LPS modification associated with Enterobacteriaceae biofilms corresponds to lipid A palmitoylation.
The presence of the additional band in LPS extracted from rough E. coli K-12, and enteropathogenic E. coli strains 55989 and O42 and uropathogenic 536 and CFT073 smooth E. coli strains (with O antigen) suggested that the modification occurs in the smallest common LPS part in all tested strains, corresponding to lipid A and the inner core. Moreover, detection of the additional band in LPS extracted from 96-h biofilms formed by E. coli K-12 BW25113 waaC deep rough mutant suggested that the biofilm-associated modification could take place on lipid A bound to a 3-deoxy-d-manno-octulosonic acid (Kdo) disaccharide part of the LPS (lipid A-Kdo2) (Fig. 2A and B). Of the E. coli enzymes potentially involved in chemical modifications of the lipid A-Kdo2 portion, four are common to E. coli 55989, O42, 536, CFT073, and K-12 strains: ArnT (amino-arabinose addition to lipid A), EptA (phosphoethanolamine addition to lipid A), EptB (phosphoethanolamine addition to Kdo) and PagP (palmitate addition to lipid A) (17). We analyzed LPS extracted from planktonic (24-h) and biofilm (96-h) bacteria corresponding to arnT, eptA, eptB, and pagP E. coli mutants and detected the biofilm-associated LPS band in biofilm formed by all mutants except the pagP mutant (data not shown and Fig. 2B). Complementation of the E. coli pagP mutant by a plasmid expressing pagP restored LPS modification in 96-h biofilm bacteria (Fig. 2B). These results suggested lipid A palmitoylation in biofilm bacteria (18). Matrix-assisted laser desorption ionization−time of flight mass spectrometry (MALDI-TOF MS) analysis of lipid A in 24-h planktonic bacteria showed one major peak at m/z = 1,797.4, corresponding to a hexa-acylated nonpalmitoylated lipid A species and only a minor species at m/z = 2,035.6, corresponding to a hepta-acylated palmitoylated lipid A species (Fig. 3A and B). In contrast, the spectrum obtained for 96-h biofilm showed two major peaks, at m/z = 1,797.4 and m/z = 2,035.6, thereby indicating significant lipid A palmitoylation under biofilm conditions. We did not detect palmitoylated lipid A in E. coli ΔpagP biofilm bacteria (Fig. 3D), while production of palmitoylated lipid A was restored upon complementation by plasmid pPagP under planktonic and biofilm conditions (Fig. 3E and F). MALDI-TOF MS analysis of LPS extracted from E. coli waaC deep rough (Re) mutant grown under planktonic condition already showed significant lipid A palmitoylation, potentially due to increased membrane stress-dependent pagP expression (19). However, comparison between planktonic and biofilm bacteria showed that the only detected modification occurring in biofilm is a difference at m/z = 2,035 and m/z = 2,475 peaks, corresponding to palmitoylated lipid A and palmitoylated lipid A-Kdo2, respectively (see Fig. S3 in the supplemental material). Finally, biofilm-associated lipid A palmitoylation is a general feature, since MALDI-TOF mass spectrometry analysis of lipid A extracted from biofilms formed by several pathogenic E. coli strains as well as various Gram-negative bacteria, including Serratia marcescens, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Citrobacter koseri species, consistently showed increased levels of lipid A palmitoylation compared to corresponding planktonic cultures (Table 1 and Fig. S4A to S4D).
TABLE 1 .
Bacterial species | Palmitoylation levelb |
Bf/Pk ratioc | |
---|---|---|---|
Pk | Bf | ||
E. coli strains | |||
K-12 BW25113 | 0.1 | 0.6 | 6 |
K-12 BW25113 ΔwaaC | 0.94 | 1.35 | 1.4 |
55989 | 0.3 | 1.1 | 3.7 |
O42 | 0.62 | 1.3 | 2.1 |
536 | 1 | 1.35 | 1.35 |
CFT073 | 0.96 | 1.72 | 1.8 |
Citrobacter koseri | 1.2 | 2.7 | 2.3 |
Serratia marcescens SM365 | 2.4 | 4.3 | 1.8 |
Klebsiella pneumoniae strains | |||
LM21 | 0.6 | 1.4 | 2.3 |
CH994 | 0.9 | 1.5 | 2.3 |
CH995 | 0.6 | 1.4 | 1.6 |
CH996 | 0.6 | 1.2 | 2.3 |
Pseudomonas aeruginosa strains | |||
PA14 | 0.12 | 0.16 | 1.3 |
BJN8 | 0.23 | 0.5 | 2.17 |
BJN33 | 0.6 | 1.1 | 1.83 |
BJN53 | 0.1 | 0.34 | 3.4 |
Some of the corresponding spectra can be found in Fig. 2 and in Fig. S3 and S4 in the supplemental material.
The palmitoylation level was determined as the peak area of the major palmitoylated species, normalized by the peak area of the corresponding nonpalmitoylated species (Fig. S4).
Ratio of the palmitoylation level in biofilm to the palmitoylation level in planktonic conditions, with all other conditions identical in the analysis.
H-NS represses pagP transcription in planktonic cultures.
To investigate regulation of lipid A palmitoylation in biofilm, we introduced a lacZ transcriptional fusion downstream of the pagP stop codon in E. coli BW25113 (transcriptional operon fusion) and observed a 3-fold increase in β-galactosidase activity in 96-h biofilm compared to planktonic culture (Fig. 4A). Moreover, constitutive expression of pagP in E. coli BW25113 PcL-pagP led to production of the additional LPS band under both planktonic and biofilm conditions (Fig. 4B), suggesting that lipid A palmitoylation is regulated at the transcriptional level. Previous studies involved PhoP and EvgA as positive regulators of pagP expression in response to magnesium limitation (19, 20); however, deletion in these 2 genes had no effect on pagP expression or on the LPS profile, demonstrating that biofilm-associated palmitoylation is PhoP and EvgA independent (Fig. 4A and C). Interestingly, the emergence of the additional LPS band under very late stationary-phase conditions (96 h of planktonic culture) is also PhoP and EvgA independent and is not inhibited upon supplementation with excess magnesium in the culture medium (see Fig. S5A in the supplemental material). Moreover, inactivation of various stress response regulators, including cpxR, rcsB, pspF, oxyR, soxR, arcA, rpoS, relA, and luxS, had no impact on lipid A palmitoylation (Fig. S5B). To identify regulators of pagP expression in E. coli, we took advantage of the white color displayed by E. coli BW25113 pagP-lacZ colonies on 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-Gal) agar plates, and we used TnSC189 mariner-based transposon mutagenesis to screen for blue BW25113 pagP-lacZ mutants derepressed for pagP expression. We identified 7 dark blue colonies corresponding either to trivial TnSC189 insertion upstream of the pagP-lacZ fusion or to insertion into the hns gene, which encodes the global silencing protein repressor H-NS (21). To confirm the role of H-NS in pagP expression, we monitored pagP expression in E. coli BW25113 Δhns pagP-lacZ planktonic cultures and observed 18-fold-increased pagP expression in the hns deletion mutant compared to the parental strain (Fig. 4A). Consistent with this result, the previously biofilm-associated LPS band could be detected in 24-h planktonic bacteria in LPS extracted from E. coli Δhns pagP-lacZ mutant (Fig. 4C). Since the ability of this mutant to form a biofilm was strongly affected, we could not meaningfully monitor pagP expression or LPS palmitoylation in biofilms. Complementation of the Δhns pagP-lacZ mutant with plasmid pBAD33-hns decreased expression of pagP (Fig. S6A), therefore demonstrating that H-NS represses lipid A palmitoylation in E. coli planktonic bacteria.
The anti-H-NS factor SlyA activates pagP transcription in mature biofilms.
Increased pagP expression under mature biofilm conditions suggested potential alleviation of H-NS repression by another E. coli regulator. Analysis of the pagP promoter region actually revealed three sequences closely matching the proposed consensus binding site for SlyA, an anti-H-NS factor that antagonizes H-NS binding on a number of cell envelope E. coli genes (see Fig. S6B in the supplemental material) (22–25). We therefore tested the contribution of SlyA to pagP regulation and compared pagP expression in E. coli BW25113 pagP-lacZ and BW25113 ΔslyA pagP-lacZ both under planktonic and biofilm conditions. We observed that a slyA deletion very significantly reduced pagP induction in biofilm, with only a 1.3-fold induction of pagP expression in the ΔslyA background, compared to 2.9-fold induction observed in the wild-type (WT) background (Fig. 4A). Consistent with this result, analysis of LPS extracted from the ΔslyA mutant showed only a minor modification of the LPS profile between planktonic and biofilm conditions (Fig. 4C). However, complementation of the slyA mutation in E. coli ΔslyA pagP-lacZ mutant with plasmid pCA24N-slyA partially restored biofilm-associated induction of pagP expression (Fig. S6C). Finally, we compared slyA expression under planktonic and biofilm conditions, and we observed a 1.7-fold induction of slyA expression in biofilm, therefore confirming the role of SlyA in biofilm-associated pagP expression (Fig. 4D).
Lipid A palmitoylation increases in vivo survival of biofilm bacteria.
To investigate the role of lipid A palmitoylation in biofilm bacteria, we first compared E. coli wild-type and ΔpagP and PcL-pagP mutant capacities to form in vitro biofilm on microtiter plates or continuous-flow microfermentors. We observed that neither the lack of, nor constitutive PagP-dependent palmitoylation, affected commensal E. coli BW25113 or pathogenic E. coli 55989 in vitro biofilm formation (see Fig. S7A and S7B in the supplemental material). We then used our previously described in vivo rat model of biofilm-associated infection in a totally implanted venous access port (TIVAP) (26) to compare the extent of in vivo biofilm development of bioluminescent wild-type, ΔpagP, or PcL-pagP E. coli 55989 derivatives in an inoculated TIVAP (Fig. 5A). Monitoring of bioluminescent biofilm biomass formed in an implanted TIVAP during the 7 days of the experiment showed a decrease in luminescence in the TIVAP colonized by E. coli 55989 ΔpagP compared to wild-type and PcL-pagP strains (Fig. 5B and C). However, inoculated TIVAP showed no initial difference in CFU recovered 3 h after inoculation, indicating that the lack of lipid A palmitoylation has no effect on initial adhesion on TIVAP in vivo (Fig. S7C). Nevertheless, we observed a significant decrease in 55989 ΔpagP bacterial number at day 7 compared to TIVAP colonized with wild-type 55989 bacteria (Fig. 5D). Since in vivo formation of E. coli 55989 biofilm in the chamber of the implanted device also led to PagP-dependent modifications of the LPS profile (Fig. 5E), we investigated the potential contribution of lipid A palmitoylation to control of biofilm infection dynamics by the host.
Lipid A palmitoylation in E. coli was previously shown to decrease the inflammatory activity of lipid A (27). To test in a controlled manner whether nonpalmitoylated E. coli 55989 ΔpagP lipid A triggers a higher inflammatory response than palmitoylated biofilm bacteria, we injected E. coli 55989 wild-type ΔpagP and PcL-pagP biofilm bacteria intravenously into rats. Two hours after injection, we used rat serum enzyme-linked immunosorbent assay (ELISA) to measure the amount of interleukin-6 (IL-6) proinflammatory cytokine and observed a significant increase in the IL-6 level induced by E. coli 55989 ΔpagP biofilm bacteria compared to wild-type and PcL-pagP bacteria (Fig. 6A). We consistently observed a 2-fold reduction in the release of IL-6 by the macrophage when brought into contact with bacteria producing palmitoylated lipid A (Fig. 6B). Moreover, palmitoylated E. coli biofilm bacteria also displayed resistance to the antimicrobial peptide protegrine-1 (PG-1) compared to unpalmitoylated bacteria (see Fig. S8 in the supplemental material). Taken together, these results showed that lipid A palmitoylation increased biofilm bacteria survival in vivo, potentially by damping the inflammatory response to and host immune defenses against biofilm bacteria.
DISCUSSION
LPS is a major structural component of the outer membrane of the Gram-negative bacterial envelope composed of three covalently linked domains, including the lipid A (or endotoxin) hydrophobic anchor, the core region, and the O-antigen polymer (28). LPS size and composition are highly dynamic and vary according to the strain and growth conditions, contributing to bacterial adaptation to changing environments (29). Here we identified a new biofilm phenotype corresponding to a high level of lipid A palmitoylation in biofilms formed by various Gram-negative bacteria and depending on the outer membrane β-barrel palmitoyl transferase PagP.
Regulation of lipid A palmitoylation was previously studied as a response to a variety of host factors or conditions and shown to be both transcriptional and posttranslational (19, 30–32). Indeed, the PagP enzyme can remain dormant until its phospholipid substrates reach the external leaflet of the outer membrane, where the PagP active site is located. Migration of phospholipids to the external leaflet occurs in response to a variety of membrane perturbations, including antimicrobial peptides, chelating agents, or temperature shift (32). Alternatively, pagP transcription can also be induced in response to several cues, for instance to magnesium limitation, triggering the PhoP-PhoQ two-component regulatory system (32, 33).
Whereas the basal level of lipid A palmitoylation in E. coli LPS is very low in planktonic batch cultures (31), here, we identified biofilm as an environment naturally inducing palmitoylation of lipid A. We show that the PhoPQ system is not involved in biofilm-associated PagP-dependent lipid A palmitoylation, which is also not inhibited upon supplementation with excess magnesium in the culture medium. In contrast, we show that pagP expression is repressed by H-NS and that biofilm-associated induction of SlyA, a DNA binding protein that inhibits H-NS activity, leads to pagP derepression. Although the signal-inducing slyA and pagP expression in mature biofilm formed under continuous culture conditions is as yet unknown, we observed that lipid A palmitoylation was also SlyA and H-NS dependent and PhoP and EvgA independent in very late stationary-phase cultures grown over long incubation time (48 h and beyond). Interestingly, regular medium renewal in these extended planktonic cultures very significantly reduced lipid A palmitoylation. This suggests that lipid A palmitoylation could be induced by extreme physicochemical conditions present in both biofilm and very late stationary-phase planktonic cultures. Such conditions could correspond to general stress induced within inner biofilm layers, potentially including major nutrient starvation or microaerobic conditions. In this study, we showed, however, that inactivation of various envelope stress and general response regulators, including cpxR, rcsB, pspF, oxyR, soxR, arcA, rpoS, relA, and luxS, had no impact on lipid A palmitoylation. Alternatively, slyA and resulting pagP induction could also be triggered by the production of yet uncharacterized metabolites or a physiological process produced within biofilm and very late stationary-phase planktonic cultures. The contribution of other factors or conditions inducing lipid A palmitoylation in biofilm is under investigation.
Lipid A palmitoylation is known to stabilize the LPS outer membrane leaflet by increasing hydrophobic interactions between neighboring LPS molecules (34). Hence, palmitoylation of lipid A could correspond to an adaptation to LPS destabilization potentially occurring under biofilm conditions wherein bacteria are subjected to various physical and chemical stresses (7, 35). However, we showed that lipid A palmitoylation does not contribute to the ability of E. coli to form biofilms in vitro. Although the biofilm biomass of a PcL-pagP strain that constitutively synthesizes palmitoylated lipid A is not significantly altered, measures were variable compared to those of the wild-type strain. This difference could indicate that deregulation of pagP expression and lipid A palmitoylation could be slightly detrimental for biofilm formation. In contrast, the absence of pagP reduces biofilm formation in a clinically relevant in vivo model of device infection in rat (26, 36). Palmitoylation of lipid A was shown to attenuate the Toll-like receptor 4 (TLR4)-mediated inflammatory response induced by lipid A (37). This is consistent with the fact that biofilm bacteria with palmitoylated lipid A also displayed decreased cytokine responses in macrophage culture cell lines and in vivo. Our results therefore suggest that lipid A palmitoylation protects biofilm bacteria from the host immune response and thus contributes to the general tolerance of bacterial biofilms during infections.
A biofilm is a highly heterogeneous environment in which bacteria undergo phenotypic differentiation, raising the question of the distribution of bacteria with palmitoylated lipid A within the biofilm population. Since our lipid A analyses were performed on all bacteria composing the biofilm, they likely reflect the average level of palmitoylation within a population composed of palmitoylated and nonpalmitoylated bacteria. Use of increased pagP and slyA expression as reporter tools will help clarify the spatial patterns of palmitoylation within biofilm microniches or layers.
Biofilm bacteria shed from colonized devices are sources of systemic bloodstream infections (38). In the absence of efficient methods to treat biofilms, replacement of contaminated devices is required in many clinical situations in order to prevent biofilm-associated infections (39, 40). Removal of implanted devices suspected of infection constitutes a difficult therapeutic decision, based as much on the nature of the pathogen colonizing the device than on actual evidence for the presence of biofilms (5). Currently, there is no routine approach to demonstrate the presence of biofilms on medical devices, and development of simplified detection methods could therefore assist clinicians in evaluating the extent of biofilm-associated risk of medical device infections (5). Our findings suggest that monitoring the lipid A palmitoylation level could be used as an indicator of the presence of high-density population of Gram-negative bacterial pathogens. While sensitivity and specificity issues will need to be addressed, we are currently evaluating whether clinical samples withdrawn from totally implantable venous access port of infected patients can be used to detect lipid A palmitoylation using immunoassay or mass spectrometry.
We showed that increased lipid A palmitoylation could be a widespread characteristic of Gram-negative bacterial biofilms. While the pagP gene is present in all sequenced E. coli and enterobacterial genomes, sequence analyses indicate that PagP homologs are also present in numerous other Proteobacteria, especially beta- and gammaproteobacteria. Interestingly, P. aeruginosa has a divergent homolog of enterobacterial PagP, and the analysis of four clinical strains showed increased level of lipid A palmitoylation in biofilms. Consistently, cystic fibrosis (CF) isolates that form biofilms in the lungs of CF patients also display palmitoylated lipid A, while isolates from patients with other conditions and isolates from the environment do not (41, 42). Interestingly, Salmonella enterica serotype Typhimurium PagP enzyme was also recently shown to palmitoylate outer membrane glycerophospholipids and generate triacylated palmitoyl-glycerophospholipids (43). This therefore suggests that increased PagP activity in biofilms could also lead to increased palmitoylation of glycerophospholipids.
In conclusion, we showed that, in addition to known characteristic properties of biofilm bacteria, including tolerance to stress, 3-dimensional architecture, and production of extracellular matrix components, increased level of PagP activity leading to palmitoylation remodeling of lipid A constitutes a new biofilm-associated phenotype potentially contributing to resistance of Gram-negative bacterial biofilms to stress and host immune responses.
MATERIALS AND METHODS
Ethics statement.
Animals were housed in the Institut Pasteur animal facilities, accredited by the French Ministry of Agriculture for performing experiments on live rodents (permit A-75-1061). Work on animals was performed in compliance with French and European regulations on care and protection of laboratory animals (European Commission directive 2010/63; French law 2013-118, 6 February 2013). The protocols used in this study for the animal model, catheter placement, in vivo biofilm formation, and in vivo study of inflammatory responses were approved by the ethics committee of “Paris Centre et Sud no. 59” under reference no. 2012-0045.
Bacterial strains and growth conditions.
Bacterial strains used in this study are described in Table S1 in the supplemental material. Antibiotics were used at the following concentrations: kanamycin, 50 µg/ml; chloramphenicol, 25 µg/ml; ampicillin, 100 µg/ml; and zeocin, 50 µg/ml. Biofilm formation in continuous-flow microfermentors was performed as previously described in reference 16. Briefly, continuous-flow microfermentors containing a removable glass spatula were used as described (http://www.pasteur.fr/recherche/unites/Ggb/matmet.html) to maximize biofilm development and minimize planktonic growth. Inoculation was performed by dipping the glass spatula for 2 min in a culture adjusted to an optical density at 600 nm (OD600) of 1 from overnight bacterial cultures grown in M63B1 minimal medium [KH2PO4 100 mM, (NH4)2SO4 15 mM, MgSO4 0.8 mM, vitamin B1 3 µM, pH 7.4] supplemented with 0.4% glucose and the appropriate antibiotics. The spatula was then reintroduced into the microfermentor, and biofilm culture was performed at 37°C in M63B1 with glucose. Biofilm biomass produced at different time points was rapidly resuspended in 15 ml of microfermentor medium (OD600 < 0.01), and biofilm bacterial LPS was analyzed after centrifugation and elimination of the resuspension supernatant.
Planktonic and biofilm cultures were performed in M63B1 medium supplemented with 0.4% glucose at 37°C.
Transposon mutagenesis, strain construction, and molecular techniques.
TnSC189 mariner-based transposon of E. coli pagP-lacZ was performed as described in reference 44. Transposon insertion sites were determined as described in reference 45. Constitutive expression of the pagP gene was carried out by insertion of the previously described ampPcL genetic element in front of pagP at its native chromosomal location, leading to the constitutive expression of the pagP gene from the phage lambda constitutive promoter (λPr) (23). Insertion of the ampPcL cassette and deletion of the pagP gene were performed using the λ-Red recombinase gene replacement system and a three-step PCR procedure described previously (46, 47). The primers used are listed in Table S2 in the supplemental material. When necessary, the antibiotic resistance marker of the inserted cassette was removed using the flippase-encoding pCP20 plasmid. For construction of pagP-lacZ and slyA-lacZ fusions, the same principle was used. The lacZ-zeo cassette was inserted downstream of the stop codon of the target gene. To construct pagP-lacZ derivatives, mutations were transferred by P1vir transduction from Keio collection E. coli JW1116 (ΔphoP), JW2366 (ΔevgA), JW1225 (Δhns), and JW5267 (ΔslyA) (48) mutants into the E. coli BW25113 pagP-lacZ strain. All constructs were confirmed by PCR and sequencing.
LPS analysis by Tricine SDS-PAGE.
Bacteria (108) were pelleted and resuspended in 100 µl of lysing buffer (Bio-Rad) containing 1% SDS, 20% glycerol, 100 mM Tris (pH 6.8), and Coomassie blue G-250. Lysates were heated at 100°C for 10 min; then, proteinase K was added at 1 mg/ml and incubated at 37°C for 1 h. These samples (4 µl) were electrophoresed in a tricine SDS-PAGE system, which improves resolution of the low-molecular-weight LPS band, using a 4% stacking gel and a 20% separating gel (49). LPSs were then visualized by the periodate-silver staining method adapted from reference 50. Gels were immersed in fixing solution (30% ethanol, 10% acetic acid) for 1 h, washed three times for 5 min each time in water, oxidized in 0.7% periodic acid for 10 min, and washed three times for 5 min each time in water. Gels were then immersed for 1 min in 0.02% thiosulfate pentahydrate, rinsed quickly in water, and stained in 25 mM silver nitrate for 10 min. After a 15-s wash in water, gels were developed in 3.5% potassium carbonate and 0.01% formaldehyde. Development was stopped in 4% Tris base and 2% acetic acid for 30 min.
Direct lipid A isolation from bacterial cells.
Lipid A was isolated directly by hydrolysis of bacterial cells as described in references 51 and 52. Briefly, 5 mg of lyophilized bacterial cells was suspended in 100 µl of a mixture of isobutyric acid−1 M ammonium hydroxide (5:3 [vol/vol]) and kept for 1.5 h at 100°C in a screw-cap test tube in a Thermomixer system. The suspension was cooled in ice water and centrifuged (2,000 × g, 5 min). The recovered supernatant was diluted with 2 volumes of water and lyophilized. The sample was then washed once with 100 µl of methanol (by centrifugation at 2,000 × g for 5 min). Finally, lipid A was extracted from the pellet in 50 µl of a mixture of chloroform, methanol, and water (3:1.5:0.25 [vol/vol/vol]).
MALDI-TOF MS analysis.
LPS samples were dispersed in water at 1 µg/µl. Lipid A extracts in chloroform-methanol-water were used directly in this mixture of solvents. In both cases, a few microliters of sample solution was desalted with a few grains of ion-exchange resin Dowex 50W-X8 (H+). Aliquots of 0.5 to 1 µl of the solution were deposited on the target, and the spot was then overlaid with matrix solution and left to dry. Dihydroxybenzoic acid (DHB) (Sigma-Aldrich) was used as the matrix. It was dissolved at 10 mg/ml in 0.1 M citric acid solution in the same solvents as those used for the analytes (53). Different analyte/matrix ratios (1:2, 1:1, and 2:1 [vol/vol]) were tested to obtain the best spectra. Negative- and positive-ion mass spectra were recorded on a PerSeptive Voyager-DE STR time of flight mass spectrometer (Applied Biosystems) in the linear mode with delayed extraction. The ion-accelerating voltage was set at [minus]20 kV, and the extraction delay time was adjusted to obtain the best resolution and signal-to-noise ratio.
β-Galactosidase activity assay.
To determine the level of β-galactosidase enzyme activity, bacteria were grown in M63B1 minimal medium supplemented with 0.4% glucose for 24 h under planktonic conditions or for 96 h under continuous-flow biofilm conditions. β-Galactosidase activity was assayed in triplicate as described previously (54) and expressed in Miller units.
Animal model. (i) Catheter placement.
Totally implanted venous access ports (TIVAPs) were surgically implanted in CD/SD (IGS:Crl) rats (Charles River) as described previously (26). Briefly, the port was implanted at dorsal midline toward the lower end of the thoracic vertebrae, and the catheter was inserted into the jugular vein. Prior to inoculation of clinical strains, all rats were checked for the absence of infection by plating 100 µl blood and by monitoring rats for the absence of luminescence signals.
(ii) TIVAP contamination in rats and in vivo biofilm formation.
The inoculum dose of 104 cells for overnight grown cultures of E. coli 55989 pAT881 wild type (WT), ΔpagP, or PcL-pagP were injected into the port in a 50-µl volume. Planktonic bacteria were removed after 3 h of injection. Progression of colonization was monitored using an IVIS100 imaging system. Rats were sacrificed either 3 h or 7 days postinjection, and TIVAPs were harvested. Serial dilutions from samples were plated on LB agar medium for enumerating CFU/ml.
(iii) In vivo inflammatory response.
To estimate the host inflammatory response due to LPS palmitoylation, E. coli 55989 WT, ΔpagP, or PcL-pagP grown in a microfermentor for 4 days were adjusted to 109 cells per 500 µl and injected into rats intravenously. Rats were sacrificed 2 h after injection, and blood was harvested aseptically and analyzed for IL-6 cytokine release in serum using ELISA.
Statistical analysis.
Two-tailed unpaired Student t test analyses were performed using Prism 5.0 for Mac OS X (GraphPad Software, Inc.). Each experiment was performed at least 3 times. Statistical significance was indicated as follows: *, P < 0.05; **, P < 0.01; ***, P < 0.001.
SUPPLEMENTAL MATERIAL
ACKNOWLEDGMENTS
We thank J.-M. Cavaillon for his interest throughout the course of this study. We thank S. Létoffé, D. Lebeaux, and O. Rendueles for critical reading of the manuscript.
This work was supported by grants from the Institut Mérieux-Institut Pasteur collaborative research program and by the French Government’s Investissement d’Avenir program, Laboratoire d’Excellence “Integrative Biology of Emerging Infectious Diseases” (grant ANR-10-LABX-62-IBEID).
Footnotes
Citation Chalabaev S, Chauhan A, Novikov A, Iyer P, Szczesny M, Beloin C, Caroff M, Ghigo J-M. 2014. Biofilms formed by Gram-negative bacteria undergo increased lipid A palmitoylation, enhancing in vivo survival. mBio 5(4):e01116-14. doi:10.1128/mBio.01116-14.
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