Published November 26, 2012 // JCB vol. 199 no. 5 831-847
The Rockefeller University Press, doi: 10.1083/jcb.201209044

Nonmedially assembled F-actin cables incorporate into the actomyosin ring in fission yeast

Video 1 Actin dynamics in LAGFP mCh-Atb2p, LAGFP, and LAmGFP cells.

Video 2 Nonmedially assembled actin cables migrate toward the middle in cdc25-22 LAGFP mCh-Atb2p cells.

Video 3 Actin cables migrated from the nonmedial region toward the middle during ring assembly in nmt1-wee1-50 adf1-1 LAGFP cells.

Video 4 TIRF video of actin dynamics in a mitotic LAGFP mCh-Atb2p cell.

Video 5 Nonmedial actin cables can also be observed in wt cells expressing Utr-CH-GFP mCh-Atb2p or Pact1-GFP-CHD.

Video 6 Nonmedial actin cables migrate toward the middle cdc25-22 Pact1-GFP-CHD mCh-Atb2p cells.

Video 7 Nonmedial actin cables migrate toward the middle in released cdc25-22 Rlc1p-3GFP LAmCh cell.

Video 8 Actin dynamics in for3Δ-related cells.

Video 9 Mitotic actin cables are either not prominent or not detectable during mitosis in cdc12Δ LAGFP mCh-Atb2p cell or for3Δ cdc12Δ LAGFP mCh-Atb2p cell.

Video 10 Single-plane videos of LAGFP mCh-Atb2p, myo2-E1 LAGFP mCh-Atb2p, and myo2-E1 myo51Δ LAGFP mCh-Atb2p cells.

Supplemental material (PDF)